A novel fluorescence assay for resveratrol determination in red wine based on competitive host-guest recognition
A novel fluorescence assay for resveratrol determination in red wine based on competitive host-guest recognition
复制标题
基于竞争性主客体识别的红酒中白藜芦醇测定的新型荧光测定法
DOI：
10.1016/j.foodchem.2018.12.133
复制
发表时间：
2019-06-15
期刊：
影响因子：
8.8
通讯作者：
Zhao, Hui
中科院分区：
文献类型：
--
作者：
Li, Can-Peng;Tan, Shuang;Zhao, Hui
本文首次建立了白藜芦醇的无标记荧光测定方法。该方法基于荧光共振能量转移（FRET），通过竞争超分子识别，在对磺化杯[6]芳烃(CX6)-改性还原氧化石墨烯（CX6@RGO）和探针-白藜芦醇配合物之间。探针分子（罗丹明B或罗丹明123）具有较强的荧光信号，其荧光基于FRET被CX6@RGO猝灭。当目标分子加入CX6@RGO时，探针分子被白藜芦醇取代，形成主客体复合物CX6@RGO-resveratrol，开启荧光信号。CX6@RGO-probe配合物的荧光强度随白藜芦醇浓度（2.0 ~ 40.0 μ M）的增加而线性增加。该方法可用于红葡萄酒中白藜芦醇的测定，检出限和回收率满意。与传统的测定方法相比，本方法具有省时、易操作、不需要样品前处理等优点。
A label-free fluorescence assay for resveratrol determination is presented for the first time. The approach was based on fluorescence resonance energy transfer (FRET), via competitive supramolecular recognition, between p-sulfonated calix[6] arene (CX6)-modified reduced graphene oxide (CX6@RGO) and a probe-resveratrol complex. The probe molecule (Rhodamine B or rhodamine 123) had a strong fluorescence signal, and its fluorescence was quenched by CX6@RGO, based on FRET. When the target molecule was added to CX6@RGO, the probe molecule was displaced by resveratrol, and a host-guest complex, CX6@RGO-resveratrol formed, turning-on the fluorescence signal. Fluorescence intensity of the CX6@RGO-probe complex increased linearly with increased resveratrol concentrations (2.0-40.0 mu M). The proposed approach was used to determine resveratrol in red wine with satisfactory detection limits and recoveries. Compared with traditional determination methods, our procedure is advantageous because it saves time, is easy to operate, and does not require sample pretreatment.