Colony-morphology screening uncovers a role for the Pseudomonas aeruginosa nitrogen-related phosphotransferase system in biofilm formation
Colony-morphology screening uncovers a role for the Pseudomonas aeruginosa nitrogen-related phosphotransferase system in biofilm formation
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DOI：
10.1111/mmi.13250
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发表时间：
2016-02-01
影响因子：
3.6
通讯作者：
Losick, Richard
中科院分区：
文献类型：
--
作者：
Cabeen, Matthew T.;Leiman, Sara A.;Losick, Richard
铜绿假单胞菌是一种机会主义的人类病原体，它的生存通过形成被称为生物膜的群落来帮助，生物膜中的细胞被包裹在自己产生的基质中。我们设计了一个基于菌落形态的突变筛选，以确定在生物膜形成中以前未被认识到的其他基因。我们的筛查发现了大多数已知的生物膜相关基因，还发现了PA14_16550和PA14_69700，它们的缺失分别抑制和增强了生物膜的形成。我们还发现编码氮调节磷酸转移酶(PTSNtr)系统的酶I的ptsP对环二GMP的产生和生物膜的形成是重要的。进一步的实验表明，在没有通过PTSNtR进行磷转移的情况下，生物膜的形成会受到阻碍，但只有在PTSNtR的调节模块--酶II(PtsN)的存在时，生物膜的形成才会受到阻碍。这些结果表明，非磷酸化的PtsN是生物被膜形成的负调控因子，并建立了PTSNtR在铜绿假单胞菌中的最早已知作用之一。
Pseudomonas aeruginosa is an opportunistic human pathogen whose survival is aided by forming communities known as biofilms, in which cells are encased in a self-produced matrix. We devised a mutant screen based on colony morphology to identify additional genes with previously unappreciated roles in biofilm formation. Our screen, which identified most known biofilm-related genes, also uncovered PA14_16550 and PA14_69700, deletions of which abrogated and augmented biofilm formation respectively. We also identified ptsP, which encodes enzyme I of the nitrogen-regulated phosphotransferase (PTSNtr) system, as being important for cyclic-di-GMP production and for biofilm formation. Further experiments showed that biofilm formation is hindered in the absence of phosphotransfer through the PTSNtr, but only in the presence of enzyme II (PtsN), the putative regulatory module of the PTSNtr. These results implicate unphosphorylated PtsN as a negative regulator of biofilm formation and establish one of the first known roles of the PTSNtr in P.aeruginosa.