Selection for antimicrobial resistance is reduced when embedded in a natural microbial community
Selection for antimicrobial resistance is reduced when embedded in a natural microbial community
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DOI：
10.1038/s41396-019-0483-z
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发表时间：
2019-12-01
期刊：
影响因子：
11
通讯作者：
Gaze, William H.
中科院分区：
文献类型：
--
作者：
Klumper, Uli;Recker, Mario;Gaze, William H.
抗生素耐药性已成为对公共卫生最紧迫的全球威胁之一。在单一物种实验中，抗生素抗性的选择发生在非常低的抗生素浓度下。然而，目前还不清楚这些发现在多大程度上可以外推到自然环境中，在那里物种嵌入复杂的群落中。我们竞争的大肠杆菌的同基因菌株，不同的唯一在一个单一的染色体耐药决定因素，在存在和不存在的猪粪便微生物群落跨越梯度的抗生素浓度为两个相关的抗生素：庆大霉素和卡那霉素。我们发现，最低选择性浓度增加了一个以上的数量级的抗生素嵌入时，在社区。我们确定了两个一般机制是负责增加最低选择浓度：增加成本的电阻和保护作用的社区易感表型。这些发现对我们理解抗生素耐药性的演变和选择具有重要意义，并可以为未来抗生素浓度的风险评估工作提供信息。
Antibiotic resistance has emerged as one of the most pressing, global threats to public health. In single-species experiments selection for antibiotic resistance occurs at very low antibiotic concentrations. However, it is unclear how far these findings can be extrapolated to natural environments, where species are embedded within complex communities. We competed isogenic strains of Escherichia coli, differing exclusively in a single chromosomal resistance determinant, in the presence and absence of a pig faecal microbial community across a gradient of antibiotic concentration for two relevant antibiotics: gentamicin and kanamycin. We show that the minimal selective concentration was increased by more than one order of magnitude for both antibiotics when embedded in the community. We identified two general mechanisms were responsible for the increase in minimal selective concentration: an increase in the cost of resistance and a protective effect of the community for the susceptible phenotype. These findings have implications for our understanding of the evolution and selection of antibiotic resistance, and can inform future risk assessment efforts on antibiotic concentrations.