Identification of a novel Pfkfb1 mRNA variant in rat fetal liver
Identification of a novel Pfkfb1 mRNA variant in rat fetal liver
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DOI：
10.1016/j.bbrc.2012.12.109
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发表时间：
2013-02-01
影响因子：
3.1
通讯作者：
Casado, Marta
中科院分区：
文献类型：
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作者：
Cosin-Roger, Jesus;Vernia, Santiago;Casado, Marta
双功能酶6-磷酸果糖-2-激酶/果糖。2，6-二磷酸酶（PFK-2/FBPase-2）催化果糖-2，6-二磷酸的合成和降解，果糖-2，6-二磷酸是葡萄糖稳态的关键代谢物。已经在哺乳动物中表征了四个基因Pfkfb 1 -4，其编码通过几个启动子和5'非编码外显子的控制通过选择性剪接产生的几种同种型。在这里，我们的特点是在胎鼠肝脏新的mRNA的变体，从一个新的Pfkfb 1基因启动子转录。长变体编码一种新的同种型（FL-PFK-2），其具有调节胎儿至成人肝脏代谢转变的相关功能。(C)2012 Elsevier Inc. All rights reserved.
The bifunctional enzyme 6-phosphofructo-2-kinase/fructose.-2,6-bisphosphatase (PFK-2/FBPase-2) catalyzes the synthesis and degradation of fructose-2,6-bisphosphate, a key metabolite in the glucose homeostasis. Four genes, Pfkfb1-4, have been characterized in mammals that code for several isoforms generated by alternative splicing through the control of several promoters and 5' non-coding exons. Here, we characterize in fetal rat liver new mRNA variants which are transcribed from a new Pfkfb1 gene promoter. The long variant codes to a new isoform (FL-PFK-2) that would be of relevant function to modulate the transition of fetal to adult liver metabolism. (C) 2012 Elsevier Inc. All rights reserved.