N-acetylcysteine reduce the stress induced by cold storage of platelets: A potential way to extend shelf life of platelets
N-acetylcysteine reduce the stress induced by cold storage of platelets: A potential way to extend shelf life of platelets
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DOI：
10.1016/j.transci.2020.103039
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发表时间：
2021-03-31
影响因子：
1.9
通讯作者：
Cho, Yong Gon
中科院分区：
文献类型：
--
作者：
Handigund, Mallikarjun;Kim, Jeong Tae;Cho, Yong Gon
全世界用于血小板的室温储存导致血小板储存损伤（PSL）和细菌生长的风险，限制了血小板的保存期限和输血安全性。因此，需要一种可用作血小板浓缩物（PC）的有效温度储存的替代储存方法。在之前的研究中，我们已经证明N-乙酰半胱氨酸（NAC）是添加剂溶液的潜在候选者，可在冷藏期间保留血小板特性长达5天。然而，该研究部分描述了疗效，并有缺点需要解决。在这里，我们使用了单采血小板产品与50 mM NAC和储存长达10天的冷藏条件下（4？一个？C）。使用Prkdcscid小鼠分析储存的血小板浓缩物的关键参数，如血小板活化、膜联蛋白V结合、唾液酸、活性氧（ROS）、神经氨酸酶活性和体内疗效。研究观察结果显示，具有NAC的PC显示血小板活化、膜联蛋白V结合、ROS产生和唾液酸水平降低。PC的体内回收显示出与储存PC相似的回收率，而与处理或储存条件无关。然而，在24小时后的第十天，室温储存的浓缩物中的回收率约为32%，而NAC处理的冷藏浓缩物中的回收率为47%。这些观察结果表明，NAC的添加在长期储存期间保护冷藏浓缩物，保持血小板的完整性。该研究还表明，延长PC储存超过10天实际上是可行的，其功效与室温（RT）储存的PC相似。
The room temperature storage used for platelets worldwide leads to platelet storage lesion (PSL) and risk of bacterial growth, limiting platelet shelf life and safety in transfusion. Thus, there is a need for an alternative storage method that can serve as effective temperature storage for platelet concentrates (PCs). In the previous investigation, we have shown that N-acetylcysteine (NAC) is a potential candidate for an additive solution to retain platelet characteristics during cold storage for up to 5 days. However, the study partially describes the efficacy and has drawbacks to address. Here, we used the apheresis platelet product with 50 mM NAC and stored up to 10 days under refrigerated condition (4 ? 1 ?C). Stored platelet concentrates were analyzed for critical parameters such as platelet activation, annexin V binding, sialic acid, reactive oxygen species (ROS), neuraminidase activity, and in vivo efficacy using Prkdcscid mice. Investigation observations revealed that PCs with NAC showed reduced platelet activation, annexin V binding, ROS production, and sialic acid levels. in vivo recovery of PCs showed similar recovery rates stored PCs irrespective of treatment or storage condition. However, on the tenth day after 24 h, recovery in room temperature stored concentrates was about 32 %, whereas in NAC treated refrigerated concentrates, it stands at 47 %. These observations indicate that NAC addition protects refrigerated concentrates during long-term storage retaining the platelet integrity. The study also suggests that extending PC storage beyond 10 days is practically accomplishable with efficacy similar to room temperature (RT) stored PCs.