Imaging CD8+ T cell dynamics in vivo using a transgenic luciferase reporter
Imaging CD8+ T cell dynamics in vivo using a transgenic luciferase reporter
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DOI：
10.1093/intimm/dxm086
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发表时间：
2007-10-01
影响因子：
4.4
通讯作者：
Crispe, Ian N.
中科院分区：
文献类型：
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作者：
Azadniv, Mitra;Dugger, Kari;Crispe, Ian N.
活化后，抗原特异性T细胞群在抗原表达位点、局部淋巴结构和其他淋巴和非淋巴器官之间流动。在这项研究中，我们记录了体内动态的CD 8（+）T细胞的抗原使用单纯疱疹病毒扩增子载体传递的反应，并揭示了几个意想不到的功能。首先，T细胞在24-48小时内定位于载体注射部位以及引流淋巴结。其次，T细胞后来重新分布的主要部位是腹腔内淋巴器官，包括乳斑、肠系膜和腰淋巴结。我们确定了各种淋巴器官中生物发光信号和抗原特异性T细胞数量之间的关系，并得出结论，生物发光信号是浅表淋巴结中T细胞丰度的有效替代指标，但在更深的结构（如脾脏）中则不然。
After activation, populations of antigen-specific T cells flow between sites of antigen expression, local lymphoid structures and other lymphoid and non-lymphoid organs. In this study, we documented the in vivo dynamics of a CD8(+) T cell response to antigen delivered using herpes simplex virus amplicon vectors and revealed several unexpected features. First, the T cells localized to the site of vector injection, as well as the draining lymph node within 24-48 h. Second, the major site to which T cells later redistributed were intra-abdominal lymphoid organs, including milky spots, mesenteric and lumbar lymph nodes. We determined the relationship between bioluminescent signal and antigen-specific T cell numbers in various lymphoid organs, and concluded that bioluminescent signal is a valid surrogate measure of T cell abundance in superficial lymph nodes, but not in deeper structures such as the spleen.