Equal parental contribution to the transcriptome is not equal control of embryogenesis.
Equal parental contribution to the transcriptome is not equal control of embryogenesis.
复制标题
父母对转录组的同等贡献并不等于对胚胎发生的同等控制。
DOI：
10.1038/s41477-020-00793-x
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Sun Meng-Xiang
中科院分区：
文献类型：
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作者：
Zhao Peng;Zhou Xuemei;Zheng Yifan;Ren Yanru;Sun Meng-Xiang
在动物中，早期胚胎发生是由母体控制的，而在植物中，父母对原胚转录组的贡献相等。因此，问题仍然是父母的早期原胚的转录组是否相等的贡献意味着早期胚胎发生的平等控制。在这里，细胞谱系特异性和等位基因特异性转录组分析的基础上，我们发现，父亲和母亲的基因组同样有助于早期原胚的顶端细胞谱系和基底细胞谱系的转录组。然而，一个强大的母源效应对基底细胞谱系的发展被发现，这表明父母的转录组的贡献是平等的，不一定再加上父母的同等控制的原胚发育。因此，不能仅仅根据父/母转录本的比例来推断父对胚胎发生的贡献。此外，我们表明，父母的起源基因显示发育阶段依赖和细胞系依赖的等位基因表达模式。这些发现将有助于调查特定的父母在早期胚胎发生的特定过程中的作用。
In animals, early embryogenesis is maternally controlled, whereas in plants, parents contribute equally to the proembryo transcriptome. Thus, the question remains whether equivalent parental contribution to the transcriptome of the early proembryo means equal control of early embryogenesis. Here, on the basis of cell-lineage-specific and allele-specific transcriptome analysis, we reveal that paternal and maternal genomes contribute equally to the transcriptomes of both the apical cell lineage and the basal cell lineage of early proembryos. However, a strong maternal effect on basal cell lineage development was found, indicating that equal parental contribution to the transcriptome is not necessarily coupled with equivalent parental control of proembryonic development. Parental contributions to embryogenesis therefore cannot be concluded solely on the basis of the ratio of paternal/maternal transcripts. Furthermore, we demonstrate that parent-of-origin genes display developmental-stage-dependent and cell-lineage-dependent allelic expression patterns. These findings will facilitate the investigation of specific parental roles in specific processes of early embryogenesis.