MicroRNA modulate alveolar epithelial response to cyclic stretch.
MicroRNA modulate alveolar epithelial response to cyclic stretch.
复制标题
DOI：
10.1186/1471-2164-13-154
复制
发表时间：
2012-04-26
期刊：
影响因子：
4.4
通讯作者：
Margulies SS
中科院分区：
文献类型：
--
作者：
Yehya N;Yerrapureddy A;Tobias J;Margulies SS
MicroRNAs (miRNAs) are post-transcriptional regulators of gene expression implicated in multiple cellular processes. Cyclic stretch of alveoli is characteristic of mechanical ventilation, and is postulated to be partly responsible for the lung injury and inflammation in ventilator-induced lung injury. We propose that miRNAs may regulate some of the stretch response, and therefore hypothesized that miRNAs would be differentially expressed between cyclically stretched and unstretched rat alveolar epithelial cells (RAECs). RAECs were isolated and cultured to express type I epithelial characteristics. They were then equibiaxially stretched to 25% change in surface area at 15 cycles/minute for 1 hour or 6 hours, or served as unstretched controls, and miRNAs were extracted. Expression profiling of the miRNAs with at least 1.5-fold change over controls revealed 42 miRNAs were regulated (34 up and 8 down) with stretch. We validated 6 of the miRNAs using real-time PCR. Using a parallel mRNA array under identical conditions and publicly available databases, target genes for these 42 differentially regulated miRNAs were identified. Many of these genes had significant up- or down-regulation under the same stretch conditions. There were 362 down-regulated genes associated with up-regulated miRNAs, and 101 up-regulated genes associated with down-regulated miRNAs. Specific inhibition of two selected miRNAs demonstrated a reduction of the increased epithelial permeability seen with cyclic stretch. We conclude that miRNA expression is differentially expressed between cyclically stretched and unstretched alveolar epithelial cells, and may offer opportunities for therapeutic intervention to ameliorate stretch-associated alveolar epithelial cell dysfunction.
登录
查看更多内容
影响因子：
2.7
作者：
Guimbellot JS;Erickson SW;Mehta T;Wen H;Page GP;Sorscher EJ;Hong JS
通讯作者：
Hong JS
影响因子：
5.5
作者：
Cavanaugh, KJ;Margulies, SS
通讯作者：
Margulies, SS
循环拉伸诱导的 miR-146a 上调通过抑制 Numb 延迟 C2C12 肌原性分化
DOI：
10.1016/j.bbrc.2008.11.041
发表时间：
2009-01-09
影响因子：
3.1
作者：
Kuang, Wei;Tan, Jiali;Liu, Yanpu
通讯作者：
Liu, Yanpu
影响因子：
8.8
作者：
dos Santos, Claudia C.;Okutani, Daisuke;Liu, Mingyao
通讯作者：
Liu, Mingyao
DOI：
10.1073/pnas.0812591106
发表时间：
2009-02-03
影响因子：
11.1
作者：
Jazdzewski, Krystian;Liyanarachchi, Sandya;De la Chapelle, Albert
通讯作者：
De la Chapelle, Albert