Structural characterization of a Delta(3), Delta(2)-enoyl-CoA isomerase from Pseudomonas aeruginosa: implications for its involvement in unsaturated fatty acid metabolism
Structural characterization of a Delta(3), Delta(2)-enoyl-CoA isomerase from Pseudomonas aeruginosa: implications for its involvement in unsaturated fatty acid metabolism
复制标题
铜绿假单胞菌 Delta(3)、Delta(2)-烯酰辅酶 A 异构酶的结构特征：对其参与不饱和脂肪酸代谢的影响
DOI：
10.1080/07391102.2018.1495102
复制
发表时间：
2019
影响因子：
4.4
通讯作者：
Bao Rui
中科院分区：
文献类型：
--
作者：
Liu Li;Li Tao;Peng Cui-Ting;Sun Chang-Zhen;Li Chang-Cheng;Xiao Qing-Jie;He Li-Hui;Wang Ning-Yu;Song Ying-Jie;Zhu Yi-Bo;Li Hong;Kang Mei;Tang Hong;Xiong Xia;Bao Rui
铜绿假单胞菌的PA4980基因编码一种可能与生物膜分散诱导信号分子-2-癸烯酸相关的enoyl-辅酶A水合酶/异构酶。为了阐明PA4980incis-2-Decenoic acid生物合成的作用，我们报道了其蛋白质产物的晶体结构。结构分析和底物结合预测表明，该酶是一种单功能的烯醇化辅酶A异构酶，可能是合成2-二十二烯酸的另一条途径。
GenePA4980fromPseudomonas aeruginosaencodes a putative enoyl-coenzyme A hydratase/isomerase that is associated with the function of the biofilm dispersion-inducing signal moleculecis-2-decenoic acid. To elucidate the role ofPA4980incis-2-decenoic acid biosynthesis, we reported the crystal structure of its protein product at 2.39 Å. The structural analysis and substrate binding prediction suggest that it acts as a monofunctional enoyl-coenzyme A isomerase, implicating an alternative pathway of thecis-2-decenoic acid synthesis.