Ghrelin modulates the immune response and increases resistance to Aeromonas hydrophila infection in hybrid tilapia
Ghrelin modulates the immune response and increases resistance to Aeromonas hydrophila infection in hybrid tilapia
复制标题
生长素释放肽调节免疫反应并提高杂交罗非鱼对嗜水气单胞菌感染的抵抗力
DOI：
10.1016/j.fsi.2020.01.006
复制
发表时间：
2020-03-01
影响因子：
4.7
通讯作者：
Zhong, Huan
中科院分区：
文献类型：
--
作者：
Han, Zhuojun;Zhou, Yi;Zhong, Huan
Ghrelin is a peptide hormone secreted by gastrointestinal tract which regulates multiple physiological processes such as appetite, metabolism, growth and gonad development in fish. In the present study, the effects of ghrelin on hybrid tilapia infected with Aeromonas hydrophila are elucidated. Juvenile hybrid tilapia fish (20.0 +/- 5.0 g) were intraperitoneally injected with 0, 0.1, 1.0, or 10.0 ng/g ghrelin/body weight synthetic ghrelin alone or in combination with A. hydrophila (0.5 x 10(6) CFU). At 10 days post treatment, the survival rate in the group that received 1.0 ng/g ghrelin/body weight ghrelin in combination with A. hydrophila was higher (66.66%) than that of the Ah group (13.33%) that received A. hydrophila alone. In tilapia that received ghrelin injections, reactive oxygen species (ROS) levels tended to increase at 5 h, while injection of 10.0 ng/g ghrelin/body weight ghrelin resulted in a significant decrease in ROS levels at 10 h. No changes in serum immune or antioxidant-related indicators were observed in fish injected with A. hydrophila compared to controls. However, ghrelin injection decreased Albumin (ALB), glutathione peroxidase (GSH-Px), lysozyme (LZM) and superoxide dismutase (SOD). Histological analysis showed that ghrelin injection alleviated the pathological changes in liver and spleen caused by A. hydrophila infection. Overall, the expression of HSP70, IL-1 beta, and TGF-beta in the liver tended to upregulate compared to the control. In the kidney, HSP70, IL-1 beta and TGF-beta levels were increased, and TNF-alpha expression levels were decreased compared to the control. The HSP70 level in the spleen was decreased, and IL-1 beta, TGF-beta, and TNF-alpha were expressed at significantly higher levels in the spleen in the tilapia that received ghrelin injections. Taken together, our results indicate that injection with 1.0 ng/g ghrelin/body weight ghrelin may effectively protect juvenile hybrid tilapia against A. hydrophila infection by improving hematological indicators, maintaining normal histology and regulating cytokine gene expression.
