Molecular cloning, characterization and expression analysis of ARMC6, ARMC7, ARMC8 from Pacific white shrimp, Litopenaeus vannamei
Molecular cloning, characterization and expression analysis of ARMC6, ARMC7, ARMC8 from Pacific white shrimp, Litopenaeus vannamei
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太平洋白虾凡纳滨对虾ARMC6、ARMC7、ARMC8的分子克隆、表征和表达分析
DOI：
10.1016/j.gene.2018.10.007
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发表时间：
2019
期刊：
影响因子：
3.5
通讯作者：
Zhang Shuang
中科院分区：
文献类型：
--
作者：
Song Shougang;Tan Beiping;Dong Xiaohui;Yang Qihui;Chi Shuyan;Liu Hongyu;Zhang Haitao;Zhang Shuang
Armadillo repeat-containing proteins （ARMCs）是一个广泛分布于真核生物中的大家族，在细胞-细胞粘附、细胞内信号传导和细胞骨架调控中发挥重要作用。本研究从凡纳滨对虾中分离到3个earmc基因termedLvARMC6、lvarmc7和lvarmc8。flvarmc6、LvARMC7和lvarmc8的完整cdna开放阅读框（ORF） (GenBank登录号：MG735126、MG728109和KX058562分别为1410 bp、570 bp和2046 bp，分别编码469、189和681个氨基酸。拓扑分析表明，LvARMC6中存在3个ARM结构域，LvARMC7中存在1个，LvARMC8中存在6个。3种LvARMCs与其他物种的同源性均在50% ~ 71%之间。系统发育分析表明，不同亚型的ARMCs形成了独立的分支，LvARMCs被分别放置在无脊椎动物的分支中，具有较强的自举支持。实时荧光定量PCR证实了flvarmcs的组成表达。LvARMC6、lvarmc7和lvarmc8分别在心脏、鳃和上皮中表达最高。在白斑综合征病毒（WSSV）、副溶血性弧菌或金黄色葡萄球菌攻击后，所有的varmcs在血细胞、肝胰腺、肠和鳃中表现出不同的表达谱。综上所述，我们的研究结果表明LvARMCs可能在先天性免疫防御中发挥作用，抵抗致病性病毒和细菌感染。
Armadillo repeat-containing proteins (ARMCs) comprise a large family that is widely distributed in eukaryotes and plays prominent roles in cell-cell adhesion, intracellular signaling, and cytoskeletal regulation. In this study, threeARMCgenes, termedLvARMC6,LvARMC7andLvARMC8, were identified and characterized from Litopenaeus vannamei. The complete cDNAs open reading frames (ORF) ofLvARMC6,LvARMC7, andLvARMC8(GenBank accession no. MG735126, MG728109 and KX058562) were 1410 bp, 570 bp and 2046 bp, encoding 469, 189, and 681 amino acids, respectively. Topology analysis indicated that three ARM domains were present in LvARMC6, one in LvARMC7 and six in LvARMC8. The identities of all the three LvARMCs with other species were between 50% and 71%. Phylogenetic analysis illustrated that different subtype of ARMCs formed their own separate branches and LvARMCs were placed in branch of invertebrates respectively with strong bootstrap support. The constitutive expressions ofLvARMCswere confirmed by real-time quantitative PCR.LvARMC6,LvARMC7andLvARMC8were expressed highest in heart, gills and epithelium, respectively. After challenge with either white spot syndrome virus (WSSV), Vibrio parahemolyticus, or Staphylococcus aureus, all of theLvARMCsdemonstrated differential expression profiles in hemocytes, hepatopancreas, intestine and gills. Taken together, our results suggest that LvARMCs may play a role in the innate immune defense against pathogenic viral and bacterial infections ofL.vannamei.