Phenotypic and functional studies of leukocytes in human endometrium and endometriosis
Phenotypic and functional studies of leukocytes in human endometrium and endometriosis
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DOI：
10.1093/humupd/4.5.702
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发表时间：
1998-09-01
影响因子：
13.3
通讯作者：
Searle, RF
中科院分区：
文献类型：
--
作者：
Jones, RK;Bulmer, JN;Searle, RF
子宫内膜异位症是一种常见的致残性疾病，其病因目前尚不清楚，尽管免疫功能障碍可使异位子宫内膜碎片在子宫腔外存活。这些研究调查了白细胞群、类固醇激素受体表达、增殖活性、子宫内膜异位症和子宫腺肌病患者不同月经周期在位和异位内膜bcl-2表达及细胞凋亡的研究异位内膜雌激素受体、bcl-2表达及CD 8+白细胞数较在位内膜明显增高，CD 56+颗粒淋巴细胞（eGL）较在位内膜明显减少，凋亡细胞在正常子宫内膜和异位内膜中均较少见。增殖活性、bcl-2表达和白细胞亚群与在位子宫内膜的关系，表明这些疾病的不同病因。通过免疫磁珠分离法可将大量存在于分泌期晚期在位子宫内膜中的不寻常的CD 56 + CD 16-eGL高度纯化（>98%）。除来自早期增殖样品的eGL的细胞毒活性可忽略不计外，来自月经周期期间非妊娠子宫内膜的eGL的细胞毒活性与外周血中的eGL相当，主要是CD 56 + CD 16+自然杀伤细胞。来自非妊娠子宫内膜和早期妊娠的eGL在48小时和120小时的时间过程中对5和100 U/ml的白细胞介素-2表现出可变的增殖反应。eGL在在位子宫内膜中显然具有重要的功能。CD+8 T细胞数量增加、雌激素受体和bcl-2表达增加可能对子宫内膜异位症的发生和发展有重要影响。
The aetiology of endometriosis, a common and disabling disorder, is presently unknown, although immune dysfunction could allow ectopic endometrial fragments to survive outside the uterine cavity, These studies investigate the relationship between leukocyte populations, steroid hormone receptor expression, proliferative activity, bcl-2 expression and apoptosis in eutopic and ectopic endometrium from women with endometriosis or adenomyosis at different phases of the menstrual cycle, Significantly increased oestrogen receptor expression, bcl-2 expression and numbers of CD8+ leukocytes were found in ectopic compared with eutopic endometrium in endometriosis, and CD56+ endometrial granulated lymphocytes (eGLs) were significantly reduced in ectopic endometrium, Apoptotic cells were rarely found in control and subject endometria, In contrast with endometriosis, adenomyotic lesions showed identical steroid hormone receptor expression, proliferative activity, bcl-2 expression and leukocyte subpopulations to eutopic endometrium, indicating different aetiologies for these disorders. The unusual CD56+ CD16- eGLs present in large numbers in late secretory phase eutopic endometrium were highly purified (>98%) by immunomagnetic separation, Except for a negligible cytotoxic activity of eGLs from early proliferative samples, cytotoxic activity of eGLs from non-pregnant endometrium during the menstrual cycle was comparable with those in peripheral blood, predominantly CD56+ CD16+ natural killer cells. eGLs from non-pregnant endometrium and early pregnancy showed a variable proliferative response to 5 and 100 U/ml interleukin-2 over 48-h and 120-h time courses. eGLs are evidently functionally important in the eutopic endometrium. Their absence in endometriotic lesions together with increased CD+8 T-cell numbers and increased oestrogen receptor and bcl-2 expression may have significant effects on the development and progression of endometriosis.