S100A4 in Spinal Substantia Gelatinosa from Dorsal Root Ganglia Modulates Neuropathic Pain in a Rodent Spinal Nerve Injury Model.
S100A4 in Spinal Substantia Gelatinosa from Dorsal Root Ganglia Modulates Neuropathic Pain in a Rodent Spinal Nerve Injury Model.
复制标题
脊神经节胶质质中的S100A4在啮齿动物脊髓神经损伤模型中调节神经病理性疼痛。
DOI：
10.2147/jpr.s293462
复制
发表时间：
2021
影响因子：
2.7
通讯作者：
Xiang Z
中科院分区：
文献类型：
--
作者：
Jiang X;Zhao W;Zhao T;Yang M;Yuan H;Qian J;Xiang Z
检测S100 A4在脊神经结扎（SNL）大鼠模型中的时空表达。同时也要弄清楚哪些其他分子直接与S100 A4相互作用，以探索可能参与神经病理性疼痛的可能机制。采用7周龄雄性SD大鼠建立SNL模型。免疫荧光和Western blotting检测S100 A4在模型中的时空表达。S100 A4与许多相关分子和可以区分细胞类型的标记分子共标记。鞘内注射S100 A4中和抗体后，记录SNL大鼠行为学变化，并比较分子变化。通过免疫共沉淀（co-IP）验证S100 A4与其他相关分子的直接相互作用，探讨其可能的作用机制。脊神经结扎后，背根神经节（DRG）和脊髓背角内S100 A4的含量显著增加。鞘内注射S100 A4中和抗体可有效缓解大鼠的机械性疼痛。co-IP揭示了S100 A4和E11之间的直接相互作用。与对照组相比，SNL大鼠背根节和脊髓背角S100 A4含量增加。鞘内注射S100 A4中和抗体可有效缓解SNL大鼠的机械性疼痛。S100 A4可能通过神经递质或其他途径参与神经病理性疼痛的产生，但具体机制有待进一步研究。
To detect the spatio-temporal expression of S100A4 in a spinal nerve ligation (SNL) rat model. Also to figure out which other molecules directly interact with S100A4 to explore the possible mechanisms which might be involved in neuropathic pain. Seven-week-old male SD rats were used for the SNL model construction. Immunofluorescence and Western blotting were used to detect the spatio-temporal expression of S100A4 in the model. S100A4 was co-labeled with a number of related molecules and marker molecules that can distinguish between cell types. After intrathecal injection of S100A4 neutralizing antibody, the behavioral changes of SNL rats were recorded, and molecular changes compared. The direct interaction between S100A4 and other related molecules was verified by co-immunoprecipitation (co-IP) to explore its possible mechanism. After spinal nerve ligation, the content of S100A4 in the dorsal root ganglion (DRG) and spinal dorsal horn increased significantly. Intrathecal injection of S100A4 neutralizing antibody could effectively relieve the mechanical pain in rats. co-IP revealed a direct interaction between S100A4 and RAGE. The content of S100A4 in the DRG and spinal dorsal horn of SNL rats increased, compared with that of the control group. Intrathecal injection of S100A4 neutralizing antibody could effectively relieve the mechanical pain in SNL rats. S100A4 may be involved in the production of neuropathic pain through RAGE or other ways, but the specific mechanism needs to be further studied.