Anatomy of the isolated area dentata grown in the rat anterior eye chamber
Anatomy of the isolated area dentata grown in the rat anterior eye chamber
复制标题
大鼠眼前房孤立区齿状体的解剖
DOI：
10.1002/cne.902080408
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发表时间：
1982
影响因子：
2.5
通讯作者：
L. Olson
中科院分区：
文献类型：
--
作者：
D. Goldowitz;A. Seiger;L. Olson
我们使用眼内移植技术来测试孤立的胎儿齿状区的发育潜力，并帮助确定脑发育的细胞排列、树突状分支、轴突生长、突触发生和连接特异性等关键方面的决定因素。从19 - 20天胚胎中取出胎儿海马结构。一个区域，称为齿状区，从海马结构解剖。该区域包括齿状回、门和内侧下区的原基。将无牙本质移植到同基因的成年大鼠的前房中。在oculo中，在最初的2个月内监测齿状区移植物的生长和血管化。移植后2 ~ 7个月，处死宿主动物，用各种组织学技术检查移植物的齿状区。绝大多数移植物的细胞结构高度地让人联想到原位的齿状区。典型的形态发生结果包括紧密堆积的、连续的、C形的颗粒细胞片，其具有苔藓状纤维，其与分离的一组分散的肺门细胞的近端树突和松散堆积的锥体细胞层接触。锥体细胞和门细胞的轴突，反过来，提供了主要的神经支配的颗粒细胞树突。颗粒细胞树突棘密度接近正常，颗粒细胞神经元内常见突触。很少发现移植物的神经支配起源于支配宿主虹膜并进入移植物的睫状神经节或三叉神经节的轴突。来自宿主虹膜的交感神经纤维以可再现的和器官型的方式支配移植物，尽管神经支配的程度小于原位齿状区的去甲肾上腺素能神经支配。发现移植物的一些Timm阳性纤维离开移植物并进入宿主虹膜。齿区移植有几个特点，这是不同于他们的原位同行。这些差异包括膨胀性颗粒细胞树突减少，纤维性星形胶质细胞的广泛存在，锥体细胞与红藻氨酸敏感性降低，过度血管化的神经元，和外周肾上腺素能纤维形态的中央特征之一的转化。
We used the intraocular transplantation technique to test the developmental potential of isolated, fetal area dentata, and to help define the determinants of such critical aspects of brain development as cell arrangements, dendritic arborization, axon growth, synaptogenesis, and connectional specificity. Fetal hippocampal formations were removed from embryos of 19 to 20 days of embryonic age. A region, termed area dentata, was dissected from the hippocampal formation. This region included the anlage of the dentate gyrus, the hilus, and the medial regio inferior. Areae dentatae were transplanted into the anterior eye chamber of isogenic, adult rats. The growth and vascularization of area dentata transplants were monitored over the first 2 months in oculo. From 2 to 7 months after transplantation host animals were killed and the area dentata transplants were examined with a variety of histological techniques. The cytoarchitectonics of a great majority of the transplants were highly reminiscent of area dentata in situ. A typical morphogenetic outcome included tightly packed, continuous, C‐shaped sheets of granule cells with mossy fibers which made contact with the proximal dendrites of a segregated group of dispersed hilar cells and a loosely packed layer of pyramidal cells. The axons of pyramidal and hilar cells, in turn, provided the major innervation of the granule cell dendrites. The granule cell dendritic spine density was nearly normal, and synapses were common in the granule cell neuropil. Very little innervation of the transplant was found to originate from the axons of the ciliary or trigeminal ganglia which innervate the host iris and enter the transplant. Sympathetic fibers from the host iris innervate the transplant in a reproducible and organotypic fashion although the degree of innervation is less than the in situ noradrenergic innervation of area dentata. Few Timm‐positive fibers of the transplant were found to leave the transplant and enter the host iris. Area dentata transplants had several features which were different from their in situ counterparts. These differences included less expansive granule cell dendrites, extensive presence of fibrous astrocytes, pyramidal cells with reduced kainic acid sensitivity, hypervascularized neuropil, and transformation of peripheral adrenergic fiber morphology to one of central characteristics.
影响因子：
1.3
作者：
Riema Abdunnur
通讯作者：
Riema Abdunnur