Kinetic markers of human embryo quality using time-lapse recordings of IVF/ICSI-fertilized oocytes
Kinetic markers of human embryo quality using time-lapse recordings of IVF/ICSI-fertilized oocytes
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DOI：
10.1016/s1472-6483(10)60222-2
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发表时间：
2008-09-01
影响因子：
4
通讯作者：
Ziebe, S.
中科院分区：
文献类型：
--
作者：
Lemmen, J. G.;Agerholm, I.;Ziebe, S.
本研究利用缩时系统研究了从受精卵到卵裂期胚胎早期发育过程中各事件发生的时间和协调性。目的是确定与优质胚胎和植入有关的标记。对102个受精卵进行了20-24 h的随访。事件，如出现和消失的（原）核和定时和同步的细胞分裂记录为受精后的时间点。计算这些事件的发生率及其同步性，并将其与受精方法、胚胎质量和植入成功率联系起来。发育成4细胞以上胚胎的受精卵母细胞比发育成3细胞或2细胞胚胎的受精卵母细胞有更早的原核消失和第一次卵裂。卵胞浆内单精子注射受精的4-细胞胚胎与IVF受精的胚胎相比，2-细胞期明显缩短（P = 0.0090）。在延时系统中的发育与在正常孵化器中培养的兄弟姐妹相似，这表明延时系统的数据可以外推到临床的实验室环境中。受精后原核的早期消失和第一次卵裂的发生与取卵后第2天较多的卵裂球有关。此外，第一次卵裂后细胞核出现的同步性与妊娠成功率显著相关（P < 0.05）。
A time-lapse system was used to study the timing and coordination of events during early development from zygote to cleavage stage embryo. The aim was to identify markers linked to good-quality embryos and implantation. A total of 102 fertilized oocytes were followed for 20-24 h. Events such as appearance and disappearance of (pro)nuclei and timing and synchronization of cell cleavage were logged as time points after fertilization. Averages for these events and their synchrony were calculated and linked with fertilization method, embryo quality and implantation success. Fertilized oocytes that developed into >= 4-cell embryos had air earlier pronuclei disappearance and first cleavage than those that developed to 3- or 2-cell embryos. Intracytoplasmic sperm injection-fertilized 4-cell embryos spent a significantly shorter period as 2-cell compared with IVF-fertilized embryos (P = 0.0090). Development in the time-lapse system was similar to their siblings cultured in normal incubators, suggesting that the data from the time-lapse system can be extrapolated to the clinic's laboratory setting. Early disappearance of pronuclei and onset of first cleavage after fertilization was correlated with a higher number of blastomeres oil day 2 after oocyte retrieval. In addition, synchrony in appearance of nuclei after the first cleavage was significantly associated with pregnancy success (P < 0.05).