Qualitative and Quantitative Analyses of the Molecular-Level Interaction between Memantine and Model Cell Membranes
Qualitative and Quantitative Analyses of the Molecular-Level Interaction between Memantine and Model Cell Membranes
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DOI：
10.1021/acs.jpcc.5b05747
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发表时间：
2015-07
影响因子：
3.7
通讯作者：
Bolin Li;Hong-Yin Wang;Peiyong Feng;Xiaofeng Han;Zhan Chen;Xiaolin Lu;Fulin Wu
中科院分区：
文献类型：
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作者：
Bolin Li;Hong-Yin Wang;Peiyong Feng;Xiaofeng Han;Zhan Chen;Xiaolin Lu;Fulin Wu
采用和频振动光谱技术（SFG），在真实的时间和原位研究了治疗阿尔茨海默病的水溶性药物美金刚（memantine）与脂双层（包括两性PC和带负电荷的PG脂双层）的分子水平相互作用。SFG结果揭示了美金刚胺如何影响这些脂质双层的脂质动力学，平均倾斜角（θ），以及角分布宽度（σ）。结果发现，美金刚可以吸附到两性离子PC表面上，但不影响的PC双层的翻转率，即使在5.0 mM的美金刚的存在下，表明美金刚和PC双层之间的相互作用可以忽略不计。然而，对于带负电荷的PG双层，发现外部PG小叶可以在相对低的美金刚浓度（1.0 mM）下被美金刚显著破坏，而内部PG小叶保持完整。此外，PG脂质外叶中CD 3群的θ和σ值也明显低于正常对照组。
Sum frequency generation (SFG) vibrational spectroscopy was employed to study the interaction between memantine (a water-soluble drug for treating Alzheimer’s disease) and lipid bilayers (including zwitterionic PC and negatively charged PG lipid bilayers) at the molecular level in real time and in situ. SFG results revealed how the memantine affected these lipid bilayers in terms of the lipid dynamics, average tilt angle (θ), as well as angle distribution width (σ). It was found that memantine could adsorb onto the zwitterionic PC surface but did not affect the flip-flop rate of the PC bilayer even in the presence of 5.0 mM memantine, indicating the negligible interaction between memantine and the PC bilayer. However, for the negatively charged PG bilayer, it was found that the outer PG leaflet could be significantly destroyed by memantine at a relatively low memantine concentration (1.0 mM), while the inner PG leaflet remained intact. Besides, the θ and σ of CD3 groups in the outer PG lipid leaflet were ...