Using oligonucleotide suspension arrays for laboratory identification of bacteria responsible for bacteremia
Using oligonucleotide suspension arrays for laboratory identification of bacteria responsible for bacteremia
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DOI：
10.1631/jzus.b0710470
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发表时间：
2008-04-01
影响因子：
5.1
通讯作者：
Chen, Zhi
中科院分区：
文献类型：
--
作者：
Hou, Xiao-li;Jiang, Han-liang;Chen, Zhi
本研究的目的是开发和验证寡核苷酸悬浮芯片，用于快速鉴定 15 种引起菌血症的细菌，尤其是在中国医院中普遍存在的菌血症。该多重阵列基于 QIAGEN LiquiChip 工作站，包括 15 个寡核苷酸探针，这些探针共价结合到不同的珠组上。细菌 23S rRNA 基因可变区的 PCR 扩增子与珠结合探针杂交。根据相应的物种特异性杂交图谱，正确鉴定了属于 15 个物种的 38 个菌株。结果表明，悬浮阵列在单次测定中可以区分多种菌株和物种的分离株，并表明悬浮阵列系统在临床实验室诊断中的潜在用途。
The aim of this study was to develop and validate an oligonucleotide suspension array for rapid identification of 15 bacterial species responsible for bacteremia, particularly prevalent in Chinese hospitals. The multiplexed array, based on the QIAGEN LiquiChip Workstation, included 15 oligonucleotide probes which were covalently bound to different bead sets. PCR amplicons of a variable region of the bacterial 23S rRNA genes were hybridized to the bead-bound probes. Thirty-eight strains belonging to 15 species were correctly identified on the basis of their corresponding species-specific hybridization profiles. The results show that the suspension array, in a single assay, can differentiate isolates over a wide range of strains and species, and suggest the potential utility of suspension array system to clinical laboratory diagnosis.