Identification of tissue kallikrein messenger RNA in human neutrophils.
Identification of tissue kallikrein messenger RNA in human neutrophils.
复制标题
人中性粒细胞中组织激肽释放酶信使 RNA 的鉴定。
DOI：
10.1007/bf02027209
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
Lundblad,RL
中科院分区：
文献类型：
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作者：
Wu,HF;Venezie,RD;Cohen,WM;Jenzano,JW;Featherstone,GL;Lundblad,RL
组织激肽释放酶在人中性粒细胞中的表达已经通过先前的使用酶和免疫化学技术的研究提出。中性粒细胞分泌这种强有力的生物因子在炎症过程中具有显著意义。本研究利用逆转录酶生成总中性粒细胞cDNA后的聚合酶链反应来证明组织激肽释放酶mRNA在人中性粒细胞中的存在。此外，使用序列特异性引物证明存在hGK-1基因的mRNA，但不存在hPK基因产物或前列腺特异性抗原基因的mRNA。这些结果证实，组织激肽释放酶存在于中性粒细胞中，并可能作为炎症过程的一部分分泌。
Expression of tissue kallikrein in human neutrophils has been suggested by previous studies using enzymatic and immunochemical techniques. Secretion of this potent biological factor by neutrophils would be of marked significance in the inflammatory process. The present study utilized the polymerase chain reaction following reverse transcriptase generation of total neutrophils cDNA to demonstrate the presence of tissue kallikrein mRNA in the human neutrophils. In addition, use of sequence-specific primers demonstrated the presence of mRNA for the hGK-1 gene, but not for the hPK gene product or the gene for prostate-specific antigen. These results confirm that tissue kallikrein is present in neutrophils and may be secreted as part of the inflammatory process.