Whole genome sequence of an edible and medicinal mushroom, Hericium erinaceus (Basidiomycota, Fungi)
Whole genome sequence of an edible and medicinal mushroom, Hericium erinaceus (Basidiomycota, Fungi)
复制标题
食用和药用蘑菇猴头菇（担子菌门，真菌）的全基因组序列
DOI：
10.1016/j.ygeno.2020.01.011
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发表时间：
2020-05-01
期刊：
影响因子：
4.4
通讯作者：
Peng, Yuande
中科院分区：
文献类型：
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作者：
Gong, Wenbing;Wang, Yahui;Peng, Yuande
猴头菌是我国著名的食用和药用菌。对这种蘑菇的生物学和遗传学研究很少，从而阻碍了优良品种的选育。在此，我们进行了从头测序和组装的H。使用Illumina和PacBio平台对猴头菌单核CS-4基因组进行测序。产生的基因组大小为41.2 Mb，N50支架大小为3.2 Mb，编码10，620个推定的预测基因。在H.刺猬。共鉴定出447个转录因子。本研究还对H.刺猬。建立了一个包含904个标记信息的微卫星标记数据库（HeSSRDb）。这些基因组资源和新设计的分子标记将丰富H.刺猬。
Hericium erinaceus is a well-known culinary and medicinal mushroom in China. The biological and genetic studies on this mushroom is rare, thereby hindering the breeding of elite cultivars. Herein, we performed de novo sequencing and assembly of H. erinaceus monokaryon CS-4 genome using the Illumina and PacBio platform. The generated genome was 41.2 Mb in size with a N50 scaffold size of 3.2 Mb, and encoded 10,620 putative predicted genes. A wide spectrum of carbohydrate-active enzymes, with the total number of 341 CAZymes, involved in lignocellulose degradation were identified in H. erinaceus. A total of 447 transcription factors were identified. This present study also characterized genome-wide microsatellites and developed markers in H. erinaceus. A comprehensive microsatellite markers database (HeSSRDb) containing the information of 904 markers was generated. These genomic resources and newly-designed molecular markers would enrich the toolbox for biological and genetic studies in H. erinaceus.