The antihistamine olopatadine regulates T cell activation in palladium allergy
The antihistamine olopatadine regulates T cell activation in palladium allergy
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DOI：
10.1016/j.intimp.2016.03.021
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发表时间：
2016-06-01
影响因子：
5.6
通讯作者：
Ogasawara, Kouetsu
中科院分区：
文献类型：
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作者：
Iguchi, Naohiko;Takeda, Yuri;Ogasawara, Kouetsu
钯(Pd)因其耐腐蚀性而被广泛应用于从时尚配饰到牙科材料的许多消费品中。然而，最近有报道称帕金森氏症过敏病例有所增加。金属过敏被归类为IV型变态反应，其特征是一种迟发型超敏反应，已知T细胞在其中发挥重要作用，但其确切作用机制尚不清楚。在这里，我们定义了组胺和PD过敏反应之间的关系，特别是关于T细胞反应。为了验证组胺对T细胞的影响，我们在体外检测了组胺或抗组胺药物盐酸奥洛他定(OLP)刺激后干扰素-γ的产生是否发生了变化。此外，我们评估了在PD过敏反应中，OLP给药是否影响了小鼠足垫肿胀的程度或干扰素-γ的产生。我们发现，组胺刺激增加了T细胞中干扰素-γ的产生，特别是与CD4(+)T细胞相比，CD8(+)T细胞中的干扰素-γ的产生更明显。有趣的是，OLP抑制CD8(+)T细胞产生干扰素-γ，该化合物抑制PD过敏小鼠的足垫肿胀和干扰素-γ的产生。这些结果表明，组胺促进了IV型变态反应，因此，组胺1受体(H1R)可能是治疗金属过敏的有用靶点。(C)2016爱思唯尔B.V.保留所有权利。
Because of its corrosion resistance palladium (Pd) has been widely used in many consumer products ranging from fashion accessories to dental materials. Recently, however, an increase in Pd allergy cases has been reported. Metal allergy is categorized as a Type IV allergy, which is characterized as a delayed-type hypersensitivity reaction in which T cells are known to play an important role; however, the precise mechanism of their action remains unclear. Here we defined the relationship between histamine and the Pd allergic reaction specifically with respect to T cell responses. To verify the effects of histamine on T cells, we examined whether there is a change in IFN-gamma production following stimulation of histamine or the antihistamine, olopatadine hydrochloride (OLP), in vitro. In addition, we assessed whether OLP administration affected the degree of footpad swelling or IFN-gamma production during the Pd allergy response in mice. We found that histamine stimulation increased IFN-gamma production in T cells, specifically enhancing IFN-gamma production in CD8(+) T cells compared with CD4(+) T cells. Interestingly, OLP suppressed the production of IFN-gamma in CD8(+) T cells, and this compound inhibited footpad swelling and IFN-gamma production in mice with Pd allergy. These results suggest that histamine promotes the Type IV allergic reaction and thus, the histamine 1 receptor (H1R) might be useful therapeutic target for treatment of metal allergy. (C) 2016 Elsevier B.V. All rights reserved.