Novel injectable biomimetic hydrogels with carbon nanofibers and self assembled rosette nanotubes for myocardial applications
Novel injectable biomimetic hydrogels with carbon nanofibers and self assembled rosette nanotubes for myocardial applications
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DOI：
10.1002/jbm.a.34400
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发表时间：
2013-04-01
影响因子：
4.9
通讯作者：
Webster, Thomas J.
中科院分区：
文献类型：
--
作者：
Meng, Xiangling;Stout, David A.;Webster, Thomas J.
本体外研究的目的是研究 pHEMA（聚甲基丙烯酸 2-羟乙酯）水凝胶中含有 RNT（花环纳米管）和 CNF（碳纳米纤维）的可注射支架上的心肌细胞功能，特别是其粘附和增殖，以确定其在心肌组织工程应用中的潜力。 RNT 是一种新型生物相容性纳米材料，由 DNA 碱基鸟嘌呤和胞嘧啶的合成类似物组装而成，当置于体温下的水溶液中时，它们会在几分钟内自组装。这些材料可以潜在地改善心肌细胞功能和 pHEMA 和 CNF 复合材料的凝固时间。由于心脏组织具有导电性，因此将 CNF 添加到 pHEMA 中以提高复合材料的导电性。我们的结果表明，心肌细胞密度在 4 小时、1 天和 3 天后增加，pHEMA 中的 CNF 和 RNT 含量更高（高达 10 mg mL1 CNF 和 0.05 mg mL1 RNT）。可能增强心肌细胞功能的因素包括更大的润湿性、电导率以及随着 CNF 和 RNT 数量的增加而增加的表面纳米粗糙度。实际上，随着 CNF 和 RNT 含量的增加，复合材料表面测量的接触角减小，而电导率和表面粗糙度增加。最后，CNF 含量较高的复合材料的极限拉伸模量会降低。总之，这些可注射复合材料的特性使其成为心肌组织工程应用的有希望的候选者，应该进一步研究。 (c) 2012 年 Wiley periodicals, Inc. J Biomed Mater Res Part A，2013 年。
The objective of the present in vitro study was to investigate cardiomyocyte functions, specifically their adhesion and proliferation, on injectable scaffolds containing RNT (rosette nanotubes) and CNF (carbon nanofibers) in a pHEMA (poly(2-hydroxyethyl methacrylate)) hydrogel to determine their potential for myocardial tissue engineering applications. RNTs are novel biocompatible nanomaterials assembled from synthetic analogs of DNA bases guanine and cytosine that self-assemble within minutes when placed in aqueous solutions at body temperatures. These materials could potentially improve cardiomyocyte functions and solidification time of pHEMA and CNF composites. Because heart tissue is conductive, CNFs were added to pHEMA to increase the composite's conductivity. Our results showed that cardiomyocyte density increased after 4 h, 1 day, and 3 days with greater amounts of CNFs and greater amounts of RNTs in pHEMA (up to 10 mg mL1 CNFs and 0.05 mg mL1 RNTs). Factors that may have increased cardiomyocyte functions include greater wettability, conductivity, and an increase in surface nanoroughness with greater amounts of CNFs and RNTs. In effect, contact angles measured on the surface of the composites decreased while the conductivity and surface roughness increased as CNFs and RNTs content increased. Lastly, the ultimate tensile modulus decreased for composites with greater amounts of CNFs. In summary, the properties of these injectable composites make them promising candidates for myocardial tissue engineering applications and should be further studied. (c) 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2013.