Facile Fabrication of Uniform Silica Films with Tunable Physical Properties Using Silicatein Protein from Sponges
Facile Fabrication of Uniform Silica Films with Tunable Physical Properties Using Silicatein Protein from Sponges
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DOI：
10.1021/la903366a
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发表时间：
2010-03-16
期刊：
影响因子：
3.9
通讯作者：
Perry, Carole C.
中科院分区：
文献类型：
--
作者：
Rai, Akhilesh;Perry, Carole C.
我们报告了一种优雅而简单的方法来制造均匀的二氧化硅薄膜，具有可控的厚度，粗糙度和亲水性，使用纳克量的silicatein，一种参与海绵中二氧化硅合成的蛋白质。通过将silicatein固定在已用胺官能化的油金涂覆表面上来实现均匀二氧化硅膜的形成。具体地，胺，胱胺和半胱胺分别具有二硫化物和硫醇基团，结合到表面上，并在蛋白质固定之前用交联剂戊二醛（GDA）处理。在环境友好的条件下，使用四甲氧基硅烷（TMOS）在硅酸盐结合表面形成二氧化硅。二氧化硅膜的厚度（20-100 nm）、粗糙度（1.2-5.2 nm）和水接触角（48度-16度）可以通过改变吸附的硅酸盐的量（10-30 ng/cm（2））和蛋白质涂覆表面暴露于二氧化硅前体的时间（30-120 min）来控制。silicatein蛋白质保留约90%的固有活性时，附着到功能化的表面，观察到类似的活性，从TMOS或四乙氧基硅烷（TEOS）形成的二氧化硅膜。这种制备可控物理性质的二氧化硅薄膜的简单路线可能在膜制造、生物医学器件、生物传感器和下一代电子元件中具有潜在的应用。
We report an elegant and simple method to fabricate uniform silica Films with controlled thickness, roughness, and hydrophilicity using nanogram quantities of silicatein, a protein involved in silica synthesis in sponges. The formation of uniform silica films was achieved by immobilization of silicatein oil gold-coated surfaces that had been functionalized with amines. Specifically, the amines, cystamine and cysteamine having disulfide and thiol groups, respectively, were bound to surfaces and treated with a cross-linking agent, glutardialdehyde (GDA) before protein immobilization. Silica was formed oil the silicatein bound surfaces under environmentally benign conditions using tetramethoxysilane (TMOS). The thickness (20-100 nm), roughness (1.2-5.2 nm), and water contact angle (48 degrees-16 degrees) of the silica Films Could be controlled by varying the amount of silicatein adsorbed (10-30 ng/cm(2)) and time of exposure of protein-coated surfaces (30-120 min) to silica precursors. The silicatein protein retained around 90% of its intrinsic activity when attached to the functionalized surfaces with similar activity being observed for silica films formed from TMOS or tetraethoxysilane (TEOS). This simple route to prepare silica films of controlled physical properties could have potential application in membrane fabrication, biomedical devices, biosensors, and next generation electronic components.