Frequent detection of human coronaviruses in clinical specimens from patients with respiratory tract infection by use of a novel real-time reverse-transcriptase polymerase chain reaction
Frequent detection of human coronaviruses in clinical specimens from patients with respiratory tract infection by use of a novel real-time reverse-transcriptase polymerase chain reaction
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DOI：
10.1086/381207
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发表时间：
2004-02-15
影响因子：
6.4
通讯作者：
Nijhuis, M
中科院分区：
文献类型：
--
作者：
van Elden, LJR;van Loon, AM;Nijhuis, M
在过去的几年中，人类冠状病毒（HCoV）已被越来越多地确定为与更严重的呼吸道感染（RTI）相关的病原体。HCoV的诊断测试在常规环境中不经常使用。因此，HCoV在RTI中发挥的确切作用可能被大大低估。我们描述了一种快速，灵敏，高特异性的实时定量逆转录聚合酶链反应（RT-PCR）检测HCoV，可以很容易地实现在常规诊断设置。在261份临床标本中，有28份（11%）检测到HCoV，这些标本来自表现出从普通感冒到严重肺炎的RTI症状的患者。从没有RTI症状的患者中获得的243份对照标本中只有1份（0.4%）显示存在HCoV。我们的结论是，HCoV可以经常检测到RTI患者。实时RT-PCR为大规模流行病学研究提供了一种工具，以进一步阐明冠状病毒感染在人类RTI中的作用。
During the past years, human coronaviruses (HCoVs) have been increasingly identified as pathogens associated with more-severe respiratory tract infection (RTI). Diagnostic tests for HCoVs are not frequently used in the routine setting. It is likely that, as a result, the precise role that HCoVs play in RTIs is greatly underestimated. We describe a rapid, sensitive, and highly specific quantitative real-time reverse-transcriptase polymerase chain reaction (RT-PCR) for the detection of HCoV that can easily be implemented in the routine diagnostic setting. HCoV was detected in 28 (11%) of the 261 clinical specimens obtained from patients presenting with symptoms of RTI ranging from common cold to severe pneumonia. Only 1 (0.4%) of the 243 control specimens obtained from patients without symptoms of RTI showed the presence of HCoV. We conclude that HCoVs can be frequently detected in patients presenting with RTI. Real-time RT-PCR provides a tool for large-scale epidemiological studies to further clarify the role that coronavirus infection plays in RTI in humans.