Diffusion tensor imaging in fixed brain tissue at 7.0 T
Diffusion tensor imaging in fixed brain tissue at 7.0 T
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DOI：
10.1002/nbm.814
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发表时间：
2003-04-01
影响因子：
2.9
通讯作者：
Lim, KO
中科院分区：
文献类型：
--
作者：
Guilfoyle, DN;Helpern, JA;Lim, KO
这项工作的目的是评估进行定量的体外脑组织扩散张量成像(DTI)测量的可行性，并检查它们与体内测量的可比性。高场强下固定组织的DTI是一种潜在的非常有价值的研究工具，因为可以实现非常高的空间分辨率。DTI被应用于人类和小鼠固定的大脑组织样本，以及对小鼠大脑的活体测量。还对固定的组织样本进行了T-1和T-2松弛成像，以提供组织的进一步特征。所有的实验都是在7T下进行的，人类固定的脑组织样本的分数各向异性(FA)在胼胝体被发现比在枕白质区域更高，这与文献中报道的活体测量一致。我们在体外和活体中对小鼠大脑的胼胝体进行的FA测量也被发现是相同的。这项初步工作支持在固定的人和动物的脑组织中使用DTI作为一种有效的研究工具。随着脑库在不同脑部疾病中的可用性增加，固定组织中的DTI可能被证明是研究白质异常的一种非常有用的方法。版权所有(C)2003 John Wiley Sons，Ltd.
The purpose of this work is to assess the feasibility of performing quantitative in vitro brain tissue diffusion tensor imaging (DTI) measurements and to examine their comparability to in vivo measurements. DTI of fixed tissue at high field strength is potentially a very valuable investigative tool as very high spatial resolution can be achieved. DTI was applied to human and mouse brain fixed tissue samples as well as in vivo measurements of the mouse brain. T-1 and T-2 relaxography of the fixed tissue samples was also performed to provide further characterization of the tissue. All experiments were performed at 7 T. The fractional anisotropy (FA) of the human fixed brain tissue samples is found to be higher in the corpus callosum than in the occipital white matter region, consistent with in vivo measurements reported in the literature. Our FA measurements of the corpus callosum of a mouse brain are also found to be the same both in vitro and in vivo. This preliminary work supports the use of DTI in both fixed human and fixed animal brain tissue as a valid investigative tool. With the increased availability of brain banks in different brain disorders, DTI in fixed tissue may prove to be a very useful method for the study of white matter abnormalities. Copyright (C) 2003 John Wiley Sons, Ltd.