RELOCALIZATION OF THE DORSAL PROTEIN FROM THE CYTOPLASM TO THE NUCLEUS CORRELATES WITH ITS FUNCTION
RELOCALIZATION OF THE DORSAL PROTEIN FROM THE CYTOPLASM TO THE NUCLEUS CORRELATES WITH ITS FUNCTION
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DOI：
10.1016/0092-8674(89)90773-3
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发表时间：
1989-12-22
期刊：
影响因子：
64.5
通讯作者：
STEWARD, R
中科院分区：
文献类型：
--
作者：
STEWARD, R
Dorsal基因是果蝇的一个具有母性活性的背腹极性基因，与脊椎动物的原癌基因c-rel同源。在野生型胚胎中，在卵裂期间在细胞质中发现背侧蛋白。在细胞核迁移到胚胎的外周后，核背蛋白的腹侧到背侧梯度被建立。核梯度的形成被破坏的突变体胚胎从其他母系活跃的背腹极性基因，在背侧化的胚胎中，只观察到胞质蛋白，而在腹侧化的胚胎的核梯度被转移到背侧。我的研究结果表明，核定位是至关重要的背作为一个形态的功能和分布的背蛋白决定细胞的命运沿着背腹轴。
Dorsal is one of the maternally active dorsal-ventral polarity genes of Drosophila and a homologous to the vertebrate proto-oncogene c-rel. In wild-type embryos, the dorsal protein is found in the cytoplasm during cleavage. After the nuclei migrate to the periphery of the embyro, a ventral-to-dorsal gradient of nuclear dorsal protein is established. The formation of the nuclear gradient is disrupted in mutant embryos from other maternally active dorsal-ventral polarity genes; in dorsalized embryos only cytoplasic protein is observed, while in ventralized embryos the nuclear gradient is shifted dorsally. My findings suggest that nuclear localization is critical for dorsal to function as a morphogen and that the distribution of the dorsal protein determines cell fate along the dorsal-ventral axis.