THE ISOLATION AND CHARACTERIZATION OF A NOVEL CDNA DEMONSTRATING AN ALTERED MESSENGER-RNA LEVEL IN NONTUMORIGENIC WILMS MICROCELL HYBRID-CELLS
THE ISOLATION AND CHARACTERIZATION OF A NOVEL CDNA DEMONSTRATING AN ALTERED MESSENGER-RNA LEVEL IN NONTUMORIGENIC WILMS MICROCELL HYBRID-CELLS
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DOI：
10.1093/nar/19.20.5763
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发表时间：
1991-10-25
影响因子：
14.9
通讯作者：
STANBRIDGE, EJ
中科院分区：
文献类型：
--
作者：
DOWDY, SF;LAI, KM;STANBRIDGE, EJ
Wilms肿瘤是一种小儿肾母细胞瘤，与11p13和11p15区域的遗传改变有关。将der（11）染色体引入G401 Wilms肿瘤细胞系中，先前已显示可恢复致瘤表型。将致瘤亲本（G401）与含有der（11）染色体的非致瘤性微细胞杂交种G401 （110.1/G401.1）进行减法cDNA/RNA杂交，鉴定出一个新的cDNA克隆，命名为QM。cDNA全长745个核苷酸，编码一种预测的亲水性25kd碱性蛋白，主要由α螺旋组成。QM转录本在多种胚胎和成人组织中表达，在成人肾脏和心脏中表达下调。QM也是一个多基因家族的成员，其成员映射到染色体6和14。QM mRNA水平在致瘤性和非致瘤性细胞系之间被调节，因此可能参与非致瘤性表型的维持。
Wilms' tumor, a pediatric nephroblastoma, has been associated with genetic alterations of the 11p13 and 11p15 regions. The introduction of a der(11) chromosome into the G401 Wilms' tumor cell line has been shown previously to revert the tumorigenic phenotype. A subtractive cDNA/RNA hybridization performed between the tumorigenic parent (G401) and a nontumorigenic microcell hybrid of G401 (110.1/G401.1) containing the der(11) chromosome resulted in the identification of a single novel cDNA clone, designated QM. The cDNA is 745 nucleotides in length and encodes a predicted hydrophilic 25 kd basic protein, primarily consisting of alpha helices. The QM transcript is expressed in a wide variety of embryonic and adult tissues and demonstrates a down regulation of expression in adult kidney and heart. QM is also a member of a multigene family members of which map to chromosomes 6 and 14. The QM mRNA level is modulated between the tumorigenic and nontumorigenic cell lines and therefore may be involved in the maintenance of the nontumorigenic phenotype.