An injectable adipose matrix for soft-tissue reconstruction.
An injectable adipose matrix for soft-tissue reconstruction.
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DOI：
10.1097/prs.0b013e31824ec3dc
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发表时间：
2012-06
影响因子：
3.6
通讯作者：
Elisseeff JH
中科院分区：
文献类型：
--
作者：
Wu I;Nahas Z;Kimmerling KA;Rosson GD;Elisseeff JH
软组织修复目前受到自体组织来源的可用性和缺乏与天然脂肪组织相当的理想软组织替代物的限制。细胞外基质（ECM）为基础的生物材料已被证明具有巨大的潜力，作为指导性的支架再生医学，机械和生物化学定义的组织起源。因此，脂肪组织独特的高脂质含量需要独特的加工条件来产生用于软组织修复的生物相容性支架。将人脂肪组织脱细胞以获得缺乏脂质和细胞的基质，同时保留ECM结构和生物活性。为了控制降解和体积持久性，使用二异氰酸酯和1-乙基-3-（-3-二甲基氨基丙基）碳二亚胺交联支架。使用人脂肪源性干细胞的体外研究来评估生物材料上的细胞活力和成脂分化。通过在小动物模型中皮下植入超过12周来评价体内生物相容性和体积持久性。该支架提供了一种生物相容性基质，支持脂肪干细胞的体外生长和分化。交联基质增加了其对酶降解的抗性。在Sprague-Dawley大鼠皮下植入脱细胞脂肪基质显示出最小的炎症反应。在植入物中观察到脂肪组织发育和血管化，宿主细胞迁移到基质中，表明基质具有指导组织重塑和再生的指导潜力。脱细胞脂肪细胞外基质具有独特的生物学和力学性能，是一种很有前途的生物支架材料，可用于同种异体软组织缺损的修复。
Soft tissue repair is currently limited by the availability of autologous tissue sources and the absence of an ideal soft tissue replacement comparable to native adipose tissue. Extracellular matrix (ECM)-based biomaterials have demonstrated great potential as instructive scaffolds for regenerative medicine, mechanically and biochemically defined by the tissue of origin. As such, the distinctive high lipid content of adipose tissue requires unique processing conditions to generate a biocompatible scaffold for soft tissue repair. Human adipose tissue was decellularized to obtain a matrix devoid of lipids and cells, while preserving ECM architecture and bioactivity. To control degradation and volume persistence, the scaffold was crosslinked using hexamethylene diisocyanate and 1-Ethyl-3-(-3-dimethylaminopropyl) carbodiimide. In vitro studies with human adipose-derived stem cells were used to assess cell viability and adipogenic differentiation on the biomaterial. In vivo biocompatibility and volume persistence were evaluated by subcutaneous implantation over 12 weeks in a small animal model. The scaffold provided a biocompatible matrix supporting the growth and differentiation of adipose-derived stem cells in vitro. Crosslinking the matrix increased its resistance to enzymatic degradation. Subcutaneous implantation of the acellular adipose matrix in Sprague-Dawley rats showed minimal inflammatory reaction. Adipose tissue development and vascularization was observed in the implant, with host cells migrating into the matrix indicating the instructive potential of the matrix for guiding tissue remodeling and regeneration. With its unique biological and mechanical properties, decellularized adipose ECM is a promising biomaterial scaffold that can potentially be used allogenically for the correction of soft tissue defects.