Genome-wide identification of cold-responsive and new microRNAs in Populus tomentosa by high-throughput sequencing
Genome-wide identification of cold-responsive and new microRNAs in Populus tomentosa by high-throughput sequencing
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DOI：
10.1016/j.bbrc.2011.12.070
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发表时间：
2012-01-13
影响因子：
3.1
通讯作者：
Wang, Yanwei
中科院分区：
文献类型：
--
作者：
Chen, Lei;Zhang, Yiyun;Wang, Yanwei
MicroRNA (miRNA) 是一种小型非编码 RNA，可调节植物生长、发育、非生物胁迫反应和病原体反应中靶标 mRNA 的表达。冷胁迫是影响植物最常见的非生物因素之一，它对植物的生长、发育和空间分布产生不利影响。为了了解 miRNA 在毛白杨寒冷胁迫下的作用，我们从经过或未经寒冷条件（4 摄氏度，8 小时）处理的幼苗中构建了两个小 RNA 文库。对这两个文库进行高通量测序，鉴定出属于 33 个 miRNA 家族的 144 个保守 miRNA 和属于 23 个 miRNA 家族的 29 个新 miRNA（及其相应的 miRNA*）。差异表达分析显示，响应冷应激，21 个 miRNA 下调，9 个 miRNA 上调。其中，19个冷响应miRNA、两个新的miRNA及其相应的miRNA*通过qRT-PCR进行了验证。使用生物信息学方法总共预测了新 miRNA 的 101 个靶基因。这些靶基因参与生长和对各种应激的抵抗。结果表明，杨树 miRNA 在冷应激反应中发挥着关键作用。 (C) 2011 Elsevier Inc. 保留所有权利。
MicroRNAs (miRNAs) are small, non-coding RNAs that regulate the expression of target mRNAs in plant growth, development, abiotic stress responses, and pathogen responses. Cold stress is one of the most common abiotic factors affecting plants, and it adversely affects plant growth, development, and spatial distribution. To understand the roles of miRNAs under cold stress in Populus tomentosa, we constructed two small RNA libraries from plantlets treated or not with cold conditions (4 degrees C for 8 h). High-throughput sequencing of the two libraries identified 144 conserved miRNAs belonging to 33 miRNA families and 29 new miRNAs (as well as their corresponding miRNA*s) belonging to 23 miRNA families. Differential expression analysis showed that 21 miRNAs were down-regulated and nine miRNAs were up-regulated in response to cold stress. Among them, 19 cold-responsive miRNAs, two new miRNAs and their corresponding miRNA*s were validated by qRT-PCR. A total of 101 target genes of the new miRNAs were predicted using a bioinformatics approach. These target genes are involved in growth and resistance to various stresses. The results demonstrated that Populus miRNAs play critical roles in the cold stress response. (C) 2011 Elsevier Inc. All rights reserved.