Lactobacillus hordniae, a New Species from the Leafhopper (Hordnia circellata)
Lactobacillus hordniae, a New Species from the Leafhopper (Hordnia circellata)
复制标题
霍德尼亚乳杆菌，叶蝉 (Hordnia circellata) 的新物种
DOI：
10.1099/00207713-27-4-362
复制
发表时间：
1977
期刊：
影响因子：
--
通讯作者：
R. Kunkee
中科院分区：
文献类型：
--
作者：
B. Latorre;C. Kado;R. Kunkee
一种革兰氏阳性杆状细菌，最初被Auger(1974)认为是葡萄皮尔斯病的病原体，通常与卷叶夜蛾有关，后者是该疾病的叶蝉媒介之一。该菌具有以下菌落和细胞特征：光滑、闪亮的白色或白垩色菌落，直径0.4-0.5 mm，整个边缘；培养5天后革兰氏阳性变得革兰氏阳性；不移动和不形成孢子；在15-37℃之间生长，最适温度在28-30℃之间，9或45℃不生长；最适生长在pH 6.5，在pH 4.5和pH 9.0下观察到生长。细菌在生长5至7天后不能存活，必须在新鲜的培养基上保持。它呈杆状(0.6x1.52.0μm)，呈3~4个细胞的短链生长，具有中体和厚度均匀的细胞壁(28~35 nm)，由电子致密的外层和类似单位膜结构的内层组成。该菌是一种兼性厌氧菌，在细胞色素氧化酶、过氧化氢酶、明胶酶、尿素酶、色氨酸酶、硝酸盐和二硫化物还原酶活性测试中呈阴性反应。蔗糖不会产生葡聚糖或黎万。它从葡萄糖和蔗糖发酵、乙酰甲基甲醇或精氨酸脱氨酶中产生L-(+)-乳酸，但不产生D-(-)-乳酸。以葡萄糖、蔗糖、半乳糖、麦芽糖、果糖、海藻糖、水杨素、菊糖和纤维二糖为碳源，不利用葡萄糖酸、乳糖、甘露糖、甘露醇、山梨醇、蜜二糖和棉子糖作为碳源。其染色体上鸟嘌呤+胞嘧啶的平均含量为32.75摩尔%。根据这些特征，该细菌似乎是乳杆菌属的一个迄今未被认识的物种，因此将其命名为Lactobacillushordniae sp.11月是提议的。模式毒株为HC-1(=ATCC 29071)。
A gram-positive, rod-shaped bacterium, originally thought by Auger (1974) to be the agent of Pierce's disease of grapevines, is commonly associated with Hordnia circellata, one of the leafhopper vectors of the disease. The bacterium has the following colonial and cellular characteristics: smooth, glistening white, or chalky colonies, 0.4 to 0.5 mm in diameter with entire margins; gram positive becoming gram variable after 5 days in culture; nonmotile and nonsporeforming; grows between 15 and 37°C with an optimum between 28 and 30°C and no growth at 9 or 45°C; optimum growth occurs at pH 6.5, with growth observed at pH 4.5 and pH 9.0. The bacterium does not survive after 5 to 7 days of growth and must be maintained on fresh medium. It is rod-shaped (0.6 by 1.5 to 2.0 μm), growing in short chains of three to four cells each, and possesses mesosomes and a cell wall of uniform thickness (28 to 35 nm) that consists of an electrondense outer layer and an inner layer resembling unit-membrane structure. The organism is a facultative anaerobe which reacts negatively in tests for cytochrome oxidase, catalase, “gelatinase,” urease, tryptophanase, and nitrate and disulfide reductase activities. No dextran or levan is produced from sucrose. It produces L-(+)-lactic acid but not D-(—)-lactic acid from glucose and sucrose fermentation, acetylmethylcarbinol, or arginine deaminase. Glucose, sucrose, galactose, maltose, fructose, trehalose, salicin, inulin, and cellobiose, but not gluconate, lactose, mannose, mannitol, sorbitol, melibiose, or raffinose, were utilized as carbon sources. Its chromosome has an average guanine-plus-cytosine content of 32.75 mol%. Based on these features, the bacterium appears to be a hitherto unrecognized species of the genus Lactobacillus, for which the name Lactobacillus hordniae sp. nov. is proposed. The type strain is HC-1 (= ATCC 29071).