Role of the community effect of cardiomyocyte in the entrainment and reestablishment of stable beating rhythms
Role of the community effect of cardiomyocyte in the entrainment and reestablishment of stable beating rhythms
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DOI：
10.1016/j.bbrc.2006.10.037
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发表时间：
2006-12-08
影响因子：
3.1
通讯作者：
Yasuda, Kenji
中科院分区：
文献类型：
--
作者：
Kojima, Kensuke;Kaneko, Tomoyuki;Yasuda, Kenji
为了研究培养心肌细胞的群体效应和夹带功能在降低搏动波动和重建同步搏动中的作用，我们建立了一种基于单细胞的二维网络培养方法，测量和比较单个细胞在形成网络前后搏动节律同步化的动态变化。通过研究两细胞网络的形成，我们发现它们的同步搏动倾向于由搏动率波动小于另一个心肌细胞的心肌细胞决定。我们进一步发现，这种趋势的强度随着网络中细胞的数量而增加。这些结果表明：（1）搏动波动是影响重建稳定的同步搏动节律的重要因素之一;（2）较大的网络减少了波动;（3）空间网络的形成本身可以稳定心肌细胞的搏动率。(c)2006年爱思唯尔公司All rights reserved.
To investigate the roles that the community effect and entrainment function of cultured cardiomyocyte play in decreasing beating fluctuation and reestablishing synchronized beating, we developed a single-cell-based two-dimensional network culture assay to measure and compare the dynamics of beating rhythm synchronization of individual cells before and after they form networks. Studying the formation of two-cell networks, we found that their synchronized beating tended to be determined by the cardiomyocyte whose beat rate fluctuated less than that of the other cardiomyocyte. We further found that the strength of this tendency increased with the number of cells in the network. These results indicate that (1) beating fluctuation is one of the important factors influencing the reestablishment of a stable synchronous beating rhythm, (2) the larger networks reduce fluctuation, and (3) the formation of a spatial network can itself stabilize cardiomyocyte beat rates. (c) 2006 Elsevier Inc. All rights reserved.