Characterization of adipose tissue-derived stromal vascular fraction for clinical application to cartilage regeneration
Characterization of adipose tissue-derived stromal vascular fraction for clinical application to cartilage regeneration
复制标题
DOI：
10.1007/s11626-014-9814-6
复制
发表时间：
2015-02-01
影响因子：
2.1
通讯作者：
Lee, Jin Woo
中科院分区：
文献类型：
--
作者：
Jang, Yeonsue;Koh, Yong Gon;Lee, Jin Woo
骨髓浓缩(BMC)是目前公认的制备间充质干细胞以促进软骨再生的方法。然而，骨髓抽吸具有高度侵入性，导致干细胞数量较少。近年来，脂肪组织来源的基质血管成分(AT-SVF)被认为是软骨再生干细胞的替代来源。然而，AT-SVF在与BMC的功能等价性方面还没有得到充分的刻画。因此，在这项研究中，我们描述了AT-SVF的特征，并评估了它作为BMC的替代方案作为一步法软骨再生手术的适宜性。AT-SVF的有核细胞数约为BMC的六分之一。然而，AT-SVF中的贴壁细胞是BMC的四倍。AT-SVF的集落形成单位频率高于BMC，分别为0.5%和0.01%。间充质干细胞(MSC)群(CD45-CD31-CD90+CD105+)在AT-SVF中占4.28%，在BMC中占0.42%；脂肪基质细胞(ASC)群(CD34+CD31-CD146-)在AT-SVF中占32%，在BMC中占0.16%。体外软骨形成实验表明，在BMC中没有形成微团，而在AT-SVF中有明显的微团形成。综上所述，未培养的AT-SVF可以作为BMC的替代物用于一步法软骨再生。
Bone marrow concentration (BMC) is the most recognized procedure to prepare mesenchymal stem cells for cartilage regeneration. However, bone marrow aspiration is highly invasive and results in low stem cell numbers. Recently, adipose tissue-derived stromal vascular fraction (AT-SVF) was studied as an alternate source of stem cells for cartilage regeneration. However, AT-SVF is not fully characterized in terms of functional equivalence to BMC. Therefore, in this study, we characterized AT-SVF and assessed its suitability as a one-step surgical procedure for cartilage regeneration, as an alternative to BMC. AT-SVF contained approximately sixfold less nucleated cells than BMC. However, adherent cells in AT-SVF were fourfold greater than BMC. Additionally, the colony-forming unit frequency of AT-SVF was higher than that of BMC, at 0.5 and 0.01%, respectively. The mesenchymal stem cell (MSC) population (CD45-CD31-CD90+CD105+) was 4.28% in AT-SVF and 0.42% in BMC, and the adipose-derived stromal cell (ASC) population (CD34+CD31-CD146-) was 32% in AT-SVF and 0.16% in BMC. In vitro chondrogenesis demonstrated that micromass was not formed in BMC, whereas it was clearly formed in AT-SVF. Taken together, uncultured AT-SVF could be used in one-step surgery for cartilage regeneration as a substitute for BMC.