Analysis of a high-throughput HLA antibody screening assay for use with platelet donors
Analysis of a high-throughput HLA antibody screening assay for use with platelet donors
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DOI：
10.1111/j.1537-2995.2008.01684.x
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发表时间：
2008-06-01
期刊：
影响因子：
2.9
通讯作者：
Stroncek, David F.
中科院分区：
文献类型：
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作者：
Fadeyi, Emmanuel;Adams, Sharon;Stroncek, David F.
背景：被动输注人类白细胞抗原抗体与输血反应有关。一种快速、廉价的对献血者进行人类白细胞抗原抗体筛查的方法可能会减少反应的发生率。研究设计与方法：用混合抗原微珠流分析仪和ELISA检测96例采集性血小板(PLT)献血者的抗-HLAI、II类抗原抗体。对于这两种检测方法，混合抗原检测反应的样品用群体反应性抗体(PRA)检测。结果：在混合抗原微球试验中，46例(48%)对I类抗原呈阳性反应，20例(21%)对II类抗原呈阳性反应。在微球PRA试验中，34例(35%)对I类抗原有抗体，18例(19%)对II类抗原有抗体，42例(44%)对I类或II类抗原有抗体。I类抗体存在于56%的女性和36%的男性。在混合抗原检测中，4份样本与I类抗原呈阳性反应，4份与II类抗原呈阳性反应，5份与I类或II类抗原呈阳性反应。5份反应样本在ELISAPRA试验中均为阳性，且均为女性。混合抗原微球试验中反应的样本在得出抗体存在的结论之前，应该通过第二次试验进行确认。
BACKGROUND: Passive infusion of HLA antibodies has been implicated in transfusion reactions. A rapid, inexpensive method of screening blood donors for HLA antibodies might reduce the incidence of reactions. A high-throughput microbead-flow analyzer HLA antibody detection technique was compared with an enzyme-linked immunosorbent assay (ELISA) method.STUDY DESIGN AND METHODS: Ninety-six apheresis platelet (PLT) donors were tested for antibodies to Class I and II HLA antigens with mixed-antigen microbead-flow analyzer and ELISAs. For both assays, samples reactive in the mixed-antigen assay were tested with a panel-reactive antibody (PRA) assay. Samples reactive in both the mixed-antigen and the PRA assays were considered positive.RESULTS: In the mixed-antigen microbead assay, 46 (48%) samples were reactive to Class I antigens and 20 (21%) to Class II. Further testing in the microbead PRA assay revealed that 34 (35%) had antibodies to Class I antigens, 18 (19%) to Class II, and 42 (44%) to either Class I or Class II. Class I antibodies were present in 56 percent of females and 36 percent of males. In the mixed-antigen ELISA, 4 samples were reactive with Class I antigens, 4 with Class II antigens, and 5 with Class I or Class II. All 5 reactive samples were also reactive in the ELISA PRA assay and were from females.CONCLUSION: The microbead assay was more sensitive than the ELISA and detected antibodies in a large proportion of donors. Samples reactive in the mixed-antigen microbead assay should be confirmed by a second assay before concluding that antibodies are present.