Generation of a homozygous ALX1 knockout human embryonic stem cell line (WAe001-A-060) by a CRISPR/Cas9 system
Generation of a homozygous ALX1 knockout human embryonic stem cell line (WAe001-A-060) by a CRISPR/Cas9 system
复制标题
通过 CRISPR/Cas9 系统生成纯合 ALX1 敲除人胚胎干细胞系 (WAe001-A-060)
DOI：
10.1016/j.scr.2021.102309
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发表时间：
2021
影响因子：
1.2
通讯作者：
Tian Zhang
中科院分区：
文献类型：
--
作者：
Xiuqing Xiao;Yashuang Chen;Lisheng Peng;Tian Zhang
人类ALX 1基因（ALX Homeobox 1）是一个蛋白质编码基因，与该基因相关的基因本体论注释包括DNA结合转录因子活性和蛋白质异二聚化活性。它是前脑间充质存活所必需的，并可能参与宫颈的发育。然而，该基因在人类中的功能尚未确定。在这里，我们通过CRISPR/Cas9系统产生了ALX 1纯合人胚胎干细胞系（WAe 001-A-060）。WAe 001-A-060在体内具有正常的未分化形态和核型、多能性和三胚层分化潜能。
Human ALX1 gene (ALX Homeobox 1) is a protein coding gene and gene ontology annotations related to this gene include DNA-binding transcription factor activity and protein heterdimerization activity. It is necessary for survival of forebrain mesenchyme and may be involved in development of cervix. However, the function of the gene has yet to be determined in humans. Here we generated an ALX1 homozygous human embryonic stem cell line (WAe001-A-060) by a CRISPR/Cas9 system. The WAe001-A-060 has a normal undifferentiated morphology and karyotype, pluripotency and three germ layers differentiation potential in vivo.