Synchronized Optical and Electronic Detection of Biomolecules Using a Low Noise Nanopore Platform
Synchronized Optical and Electronic Detection of Biomolecules Using a Low Noise Nanopore Platform
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DOI：
10.1021/nn506572r
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发表时间：
2015-02-01
期刊：
影响因子：
17.1
通讯作者：
Edel, Joshua B.
中科院分区：
文献类型：
--
作者：
Pitchford, William H.;Kim, Hyung-Jun;Edel, Joshua B.
在过去的二十年中，已经有大量的研究将纳米孔用作单分子传感器，这是受到库尔特计数器和分子在生物孔中的传输的启发。最近，增加结构分辨率和分析通量的愿望导致了额外的检测方法，如荧光光谱的整合。由于分子的高迁移速度，高带宽测量对电子探测结构信息至关重要。最常用的固态纳米孔传感器由氮化硅膜和体硅衬底组成。不幸的是，与这些平台的照明相关联的光致噪声限制了它们对高带宽、高激光功率的同步光学和电子测量的适用性。在这里，我们提出了一种独特的低噪声纳米孔平台，主要由Pyrex衬底和氮化硅膜组成，用于同步光学和电子检测生物分子。进行的原理性实验证明，Pyrex基板具有大幅降低的离子电流噪声所产生的激光照射和平台电容。此外，使用共聚焦显微镜和部分金属孔，我们证明了高信噪比同步的光学和电子检测dsDNA。
In the past two decades there has been a tremendous amount of research into the use of nanopores as single molecule sensors, which has been inspired by the Coulter counter and molecular transport across biological pores. Recently, the desire to increase structural resolution and analytical throughput has led to the integration of additional detection methods such as fluorescence spectroscopy. For structural information to be probed electronically high bandwidth measurements are crucial due to the high translocation velocity of molecules. The most commonly used solid-state nanopore sensors consist of a silicon nitride membrane and bulk silicon substrate. Unfortunately, the photoinduced noise associated with illumination of these platforms limits their applicability to high-bandwidth, high-laser-power synchronized optical and electronic measurements. Here we present a unique low-noise nanopore platform, composed of a predominately Pyrex substrate and silicon nitride membrane, for synchronized optical and electronic detection of biomolecules. Proof of principle experiments are conducted showing that the Pyrex substrates have substantially lowers ionic current noise arising from both laser illumination and platform capacitance. Furthermore, using confocal microscopy and a partially metallic pore we demonstrate high signal-to-noise synchronized optical and electronic detection of dsDNA.