Inactivation of intracellular and non-enveloped viruses by a non-ionic naphthalene endoperoxide.
Inactivation of intracellular and non-enveloped viruses by a non-ionic naphthalene endoperoxide.
复制标题
非离子萘内过氧化物灭活细胞内和无包膜病毒。
作者：
A. Dewilde;C. Pellieux;C. Pierlot;P. Wattré;J. Aubry
单线态氧（1O2，1delta（g））选择性氧化许多生物靶点，其中一些，如病毒，在体内条件下位于细胞内。考虑到1O2在水介质中的短寿命，必须在目标附近产生这种物质。因此，设计了一种1O2的水溶性和非离子载体DHPNO2，用于通过脂质膜输送1O2。与已知的仅灭活细胞外包膜病毒的阴离子载体NDPO 2相反，新化合物对所有类型的病毒，包膜（HIV）和非包膜（脊髓灰质炎病毒），细胞外和细胞内表现出杀病毒活性。HIV灭活也可以在红细胞的存在下实现，这表明DHPNO2可能用于细胞血液制品的去污。
Singlet oxygen (1O2, 1delta(g)) selectively oxidizes many biological targets, some of which, such as viruses, are located intracellularly under in vivo conditions. Considering the short lifetime of 1O2 in aqueous media, it is essential to generate this species in close proximity to the targets. Therefore, a water-soluble and non-ionic carrier of 1O2, DHPNO2, has been designed to convey 1O2 through lipid membranes. In contrast to the known anionic carrier NDPO2, which inactivates only extracellular enveloped viruses, the new compound exhibits virucidal activity on all types of viruses, enveloped (HIV) and non-enveloped (Poliovirus), extracellular and intracellular. HIV inactivation can also be achieved in the presence of red blood cells, suggesting the possible use of DHPNO2 in the decontamination of cellular blood products.