Receptor activator of nuclear factor-kappa ligand, OPG, and IGF-I expression during orthodontically induced inflammatory root resorption in the recombinant human growth hormone-treated rats.
Receptor activator of nuclear factor-kappa ligand, OPG, and IGF-I expression during orthodontically induced inflammatory root resorption in the recombinant human growth hormone-treated rats.
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DOI：
10.2319/052014-361.1
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发表时间：
2015-07
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影响因子：
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通讯作者：
Yajun Hu;Wentao Liu;Zhijian Liu;Wenying Kuang;Hong He
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文献类型：
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作者：
Yajun Hu;Wentao Liu;Zhijian Liu;Wenying Kuang;Hong He
目的探讨生长激素（GH）对正畸诱导大鼠炎症牙根吸收过程中核因子κ配体局部受体激活剂（RANKL）、OPG和IGF-I表达的影响。材料与方法40只Wistar大鼠（性别：雄性；年龄：7周）随机分为对照组和实验组。施加50g的力以将右上第一磨牙向近中移动。实验组和对照组每天分别皮下注射重组人生长激素（GH；2 mg/kg）和等体积的生理盐水。在第 1、3、7 和 14 天处死大鼠。使用微计算机断层扫描重建右上第一磨牙的图像来调查牙根吸收和牙齿移动。制备上颌骨的水平切片用于苏木精和伊红、酒石酸抗性酸性磷酸酶和免疫组织化学染色。结果 第7天和第14天，右侧第一磨牙远中颊根受压侧出现吸收陷窝。与对照组相比，GH治疗组第3天显示更多的RANKL阳性细胞和破骨细胞，第7天和第14天显示更多的OPG和IGF-I阳性细胞，以及更少的破牙细胞。治疗组牙根吸收指数较低，牙齿移动更快。 第7天和第14天GH处理组优于对照组。结论GH对强力牙根吸收的抑制作用可能是由RANKL/OPG和IGF-I介导的。短期 GH 给药可能是减少牙根吸收并缩短治疗时间的一种方法，特别是对于容易发生牙根吸收的患者。
OBJECTIVE To investigate the effects of growth hormone (GH) on local receptor activator of nuclear factor-kappa ligand (RANKL), OPG, and IGF-I expression during orthodontically induced inflammatory root resorption in rats. MATERIALS AND METHODS Forty Wistar rats (gender: male; age: 7 weeks) were randomly divided into control and experimental groups. A force of 50 g was applied to move the right upper first molars mesially. The experimental and control groups received daily subcutaneous injections of recombinant human growth hormone (GH; 2 mg/kg) and equivalent volumes of saline, respectively. The rats were sacrificed on days 1, 3, 7, and 14. Micro-computed tomography-reconstructed images of the upper right first molars were used to survey root resorption and tooth movement. Horizontal sections of the maxillae were prepared for hematoxylin and eosin, tartrate-resistant acid phosphatase, and immunohistochemical staining. RESULTS Resorption lacunae appeared on the compressed side of the distal buccal root of the right first molar on days 7 and 14. Compared with the control groups, GH-treated groups showed more RANKL-positive cells and osteoclasts on day 3 and more OPG- and IGF-I-positive cells and fewer odontoclasts on days 7 and 14. Indexes of root resorption were lower and tooth movement was faster in the GH-treated groups than in the control groups on days 7 and 14. CONCLUSIONS The inhibitory effect of GH on root resorption by heavy force might be mediated by RANKL/OPG and IGF-I. Short-term GH administration may be a method with which to reduce root resorption and shorten treatment time, especially in patients who are susceptible to root resorption.