QTL meta-analysis of root traits in Brassica napus under contrasting phosphorus supply in two growth systems.
QTL meta-analysis of root traits in Brassica napus under contrasting phosphorus supply in two growth systems.
复制标题
两种生长系统磷供应对比下甘蓝型油菜根系性状的QTL荟萃分析
DOI：
10.1038/srep33113
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发表时间：
2016-09-14
影响因子：
4.6
通讯作者：
Meng J
中科院分区：
文献类型：
--
作者：
Zhang Y;Thomas CL;Xiang J;Long Y;Wang X;Zou J;Luo Z;Ding G;Cai H;Graham NS;Hammond JP;King GJ;White PJ;Xu F;Broadley MR;Shi L;Meng J
构建了基于SNP的高密度遗传连锁图，并与先前的tapidor X Ningyou7（TNDH）甘蓝纳普斯人种群中的先前图进行了整合，从而提供了一个新的地图，其新地图总计为2041个分子标记，平均标记密度从0.39（0.39（0.82 SNP BIN））增加。使用“小袋和灯芯”系统在低和“正常”磷酸盐（PI）供应下筛选根和芽特征，并以前在基于琼脂的系统中进行了筛选。在治疗和生长系统下，P效率的母体Ningyou7的初级根长度（PRL）较短，侧根密度（LRD）较大，并且具有更大的芽生物质。定量性状基因座（QTL）分析确定了总共131个QTL，QTL荟萃分析发现了整个生长系统的四个综合QTL。集成将置信区间降低了约41％。在染色体A3上共同划分了根和芽生物量的QTL，并将侧根出现在染色体A4/C4和C8/C9上共置。 C9染色体上有一个主要的LRD QTL，解释了表型变异的18％。 LRD增加的基础QTL可能是Brassica P摄取效率的有用育种靶标。
A high-density SNP-based genetic linkage map was constructed and integrated with a previous map in the Tapidor x Ningyou7 (TNDH) Brassica napus population, giving a new map with a total of 2041 molecular markers and an average marker density which increased from 0.39 to 0.97 (0.82 SNP bin) per cM. Root and shoot traits were screened under low and ‘normal’ phosphate (Pi) supply using a ‘pouch and wick’ system, and had been screened previously in an agar based system. The P-efficient parent Ningyou7 had a shorter primary root length (PRL), greater lateral root density (LRD) and a greater shoot biomass than the P-inefficient parent Tapidor under both treatments and growth systems. Quantitative trait loci (QTL) analysis identified a total of 131 QTL, and QTL meta-analysis found four integrated QTL across the growth systems. Integration reduced the confidence interval by ~41%. QTL for root and shoot biomass were co-located on chromosome A3 and for lateral root emergence were co-located on chromosomes A4/C4 and C8/C9. There was a major QTL for LRD on chromosome C9 explaining ~18% of the phenotypic variation. QTL underlying an increased LRD may be a useful breeding target for P uptake efficiency in Brassica.