Osteoblast cell behavior on the new beta-type Ti-25Ta-25Nb alloy
Osteoblast cell behavior on the new beta-type Ti-25Ta-25Nb alloy
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DOI：
10.1016/j.msec.2012.04.042
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发表时间：
2012-08-01
影响因子：
7.9
通讯作者：
Gloriant, Thierry
中科院分区：
文献类型：
--
作者：
Cimpean, Anisoara;Mitran, Valentina;Gloriant, Thierry
在骨替代材料中，β钛合金因其低模量、高耐腐蚀性和良好的生物相容性而越来越受到重视。本研究通过评价人成骨细胞的行为，对新近开发的β型Ti-25 Ta-25 Nb合金的体外细胞相容性进行了研究。金属Ti-6Al-4V生物材料（用于生物医学应用的代表性α + β型钛合金之一）和组织培养聚苯乙烯（TCPS）也分别作为参考钛基材料和对照基材进行了研究。两种金属表面进行了分析，X射线衍射，原子力显微镜和X射线光电子能谱。通过评估活力、细胞附着和铺展、细胞形态、纤连蛋白的产生和细胞外组织以及细胞增殖来量化细胞应答。两种材料的抛光表面具有等轴晶粒显微结构和纳米级表面粗糙度，在所有分析的细胞参数方面引起了基本相同的成骨细胞反应。因此，在两个表面上，细胞显示出高存活率、良好的细胞粘附和铺展、致密且随机分散的纤连蛋白基质以及随着孵育时间增加的细胞增殖率。此外，与TCPS相比，Ti-25 Ta-25 Nb的生物学性能增强得到了结果的高度支持。这些发现，加上先前显示的超弹性行为，低杨氏模量和高耐腐蚀性，推荐Ti-25 Ta-25 Nb作为骨植入应用的良好候选材料。(c)2012 Elsevier B. V.保留所有权利。
Among metallic materials used as bone substitutes, beta titanium alloys gain an increasing importance because of their low modulus, high corrosion resistance and good biocompatibility. In this work, an investigation of the in vitro cytocompatibility of a recently new developed beta-type Ti-25Ta-25Nb alloy was carried out by evaluating the behavior of human osteoblasts. The metallic Ti-6Al-4V biomaterial, which is one of representative alpha + beta type titanium alloys for biomedical applications, and Tissue Culture Polystyrene (TCPS), were also investigated as reference Ti-based material and control substrate, respectively. Both metallic surfaces were analyzed by X-ray diffraction, atomic force microscopy and X-ray photoelectron spectroscopy. The cellular response was quantified by assessments of viability, cell attachment and spreading, cell morphology, production and extracellular organization of fibronectin and cell proliferation. Polished surfaces from both materials having an equiaxed grain microstructure and nanometre scale surface roughness elicited an essentially identical osteoblast response in terms of all analyzed cellular parameters. Thus, on both surfaces the cells displayed high survival rates, good cell adhesion and spreading, a dense and randomly dispersed fibronectin matrix and increasing cell proliferation rates over the incubation time. Furhermore, the enhanced biological performance of Ti-25Ta-25Nb was highly supported by the results obtained in comparison with TCPS. These findings, together with previously shown superelastic behavior, low Young's modulus and high corrosion resistance, recommend Ti-25Ta-25Nb as good candidate for applications in bone implantology. (c) 2012 Elsevier B.V. All rights reserved.