Basic fibroblast growth factor promotes macaque follicle development in vitro.
Basic fibroblast growth factor promotes macaque follicle development in vitro.
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DOI：
10.1530/rep-14-0557
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发表时间：
2015-05
期刊：
影响因子：
3.8
通讯作者：
Cui-ling Lu;Jie Yan;X. Zhi;Xi Xia;Tianren Wang;Liying Yan;Yang Yu;Ting Ding;Jiangman Gao;Rong Li;Jie Qiao
中科院分区：
文献类型：
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作者：
Cui-ling Lu;Jie Yan;X. Zhi;Xi Xia;Tianren Wang;Liying Yan;Yang Yu;Ting Ding;Jiangman Gao;Rong Li;Jie Qiao
生育力保存是生殖健康领域前沿科学研究的重要类型。培养卵巢皮质以i）启动原始卵泡生长和ii）获得发育中的卵泡用于以后的卵母细胞成熟是一种有前途的生育力保存策略，特别是对于老年妇女或癌症患者。目前，这一目标在灵长类动物中仍然很大程度上没有得到证实，因为通过卵巢皮质培养获得相对较大的卵泡很困难。为了克服这一障碍，我们培养猕猴卵巢皮质与FSH，试剂盒配体（KL），碱性成纤维细胞生长因子（bFGF），和/或表皮生长因子（EGF）。各种因素及因素组合对原始卵泡发育均有不同程度的促进作用。值得注意的是，bFF（bFGF，100 ng/ml和FSH，50 ng/ml）和KF（KL，100 ng/ml和FSH，50 ng/ml）均有助于在培养第12天（D12）激活原始卵泡，而在D18，bFF和KF组中发育卵泡的比例显著高于其他处理组，尤其是bFF组。bFF组的雌二醇和孕酮产量也最高，初级卵泡直径最大。直至D24，bFF组仍表现出最高比例的发育卵泡。总之，bFGF-FSH组合促进非人灵长类原始卵泡体外发育，最佳实验窗口在18天内。这些结果为人类卵巢皮质培养的未来成功以及最终获得用于生育力恢复的成熟人类卵泡或卵母细胞提供了证据。
Fertility preservation is an important type of frontier scientific research in the field of reproductive health. The culture of ovarian cortices to i) initiate primordial follicle growth and ii) procure developing follicles for later oocyte maturation is a promising fertility preservation strategy, especially for older women or cancer patients. At present, this goal remains largely unsubstantiated in primates because of the difficulty in attaining relatively large follicles via ovarian cortex culture. To overcome this hurdle, we cultured macaque monkey ovarian cortices with FSH, kit ligand (KL), basic fibroblast growth factor (bFGF), and/or epidermal growth factor (EGF). The various factors and factor combinations promoted primordial follicle development to different extents. Notably, both bFF (bFGF, 100 ng/ml and FSH, 50 ng/ml) and KF (KL, 100 ng/ml and FSH, 50 ng/ml) contributed to the activation of primordial follicles at day 12 (D12) of culture, whereas at D18, the proportions of developing follicles were significantly higher in the bFF and KF groups relative to the other treatment groups, particularly in the bFF group. Estradiol and progesterone production were also highest in the bFF group, and primary follicle diameters were the largest. Up until D24, the bFF group still exhibited the highest proportion of developing follicles. In conclusion, the bFGF-FSH combination promotes nonhuman primate primordial follicle development in vitro, with the optimal experimental window within 18 days. These results provide evidence for the future success of human ovarian cortex culture and the eventual acquisition of mature human follicles or oocytes for fertility restoration.