Transcriptional activation of ribosome-related genes at initial photoreception is dependent on signals derived from both the nucleus ans the chloroplants in Arabidopsis thaliana.
Transcriptional activation of ribosome-related genes at initial photoreception is dependent on signals derived from both the nucleus ans the chloroplants in Arabidopsis thaliana.
复制标题
初始光接收时核糖体相关基因的转录激活取决于来自拟南芥细胞核和叶绿植物的信号。
DOI：
10.1007/s10265-022-01430-8
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发表时间：
2023
期刊：
影响因子：
--
通讯作者：
M.
中科院分区：
文献类型：
--
作者：
Akagi;C.;Kurihara;Y.;Makita;Y.;Kawaguchi;M.;Tsuge;T.;Aoyama;T.;Matsui;M.
光是植物生长和发育所需的不可或缺的元素之一。特别是，它是诱导形态发生，如抑制下胚轴伸长和子叶扩张，植物经历时，他们第一次出现后发芽。然而，缺乏关于基因表达的知识，特别是在光暴露时诱导反应的翻译水平。我们研究了拟南芥幼苗在黑暗中萌发后暴露于蓝色单色光下核基因的翻译表达。在这项研究中，在蓝光受体突变体cry 1cry 2和光信号突变体5中进行核糖体分析，以了解哪些信号通路负责蓝光暴露后翻译水平的基因表达变化。分析表明，在野生型中，某些叶绿体和核糖体相关基因的表达在翻译水平上上调。然而，在这两种突变体中，核糖体相关基因的翻译上调明显受到损害。这表明通过光感受器的光信号和HY 5转录因子负责核糖体相关基因的翻译。为了进一步了解叶绿体光感受性对核基因表达的影响，通过添加光合作用抑制剂3-（3，4-二氯苯基）-1，1-二甲基脲（DCMU）和类胡萝卜素合成抑制剂氟草酮（norflurazon）来抑制叶绿体功能。结果表明，叶绿体功能的抑制并没有导致核糖体相关基因在翻译水平上的表达增加。这些结果表明，来自细胞核和叶绿体的信号是激活核糖体相关基因在蓝光接收翻译所必需的。
Light is one of the indispensable elements that plants need in order to grow and develop. In particular, it is essential for inducing morphogenesis, such as suppression of hypocotyl elongation and cotyledon expansion, that plants undergo when they first emerge after germination. However, there is a lack of knowledge about the gene expression and, in particular, the translational levels that induce a response upon light exposure. We have investigated the translational expression of nuclear genes inArabidopsis thalianaseedlings germinated in the dark and then exposed to blue monochromatic light. In this study, ribosome profiling analysis was performed in the blue-light-receptor mutantcry1cry2and the light-signaling mutanthy5to understand which signaling pathways are responsible for the changes in gene expression at the translational level after blue-light exposure. The analysis showed that the expression of certain chloroplast- and ribosome-related genes was up-regulated at the translational level in the wild type. However, in both mutants the translational up-regulation of ribosome-related genes was apparently compromised. This suggests that light signaling through photoreceptors and the HY5 transcription factor are responsible for translation of ribosome-related genes. To further understand the effect of photoreception by chloroplasts on nuclear gene expression, chloroplast function was inhibited by adding a photosynthesis inhibitor, 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU), and a carotenoid synthesis inhibitor, norflurazon. The results show that inhibition of chloroplast function did not lead to an increase in the expression of ribosome-related genes at the translational level. These results suggest that signals from both the nucleus and chloroplasts are required to activate translation of ribosome-related genes during blue-light reception.