Proteomic analysis of the response to NaCl stress of Lactobacillus bulgaricus
Proteomic analysis of the response to NaCl stress of Lactobacillus bulgaricus
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DOI：
10.1007/s10529-014-1601-7
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发表时间：
2014-07
影响因子：
2.7
通讯作者：
Chun Xing Li;Pei Li;Jinwei Sun;G. Huo;Libo Liu
中科院分区：
文献类型：
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作者：
Chun Xing Li;Pei Li;Jinwei Sun;G. Huo;Libo Liu
Lactobacillus bulgaricus is commonly used in dairy products as a starter culture. Its viability during freeze-drying is of commercial interest. Here a significant (p < 0.05) improvement in survival rate of L. bulgaricus ATCC 11842 was achieved during freeze-drying when it was prestressed with 2 % (w/v) NaCl for 2 h in the late growth phase. To understand the mechanism of this stress-related viability improvement in L. bulgaricus, protein synthesis was analyzed by 2D difference gel electrophoresis. Nine protein spots were significantly altered by NaCl and were subsequently identified by peptide mass fingerprinting. The functions of the proteins included stress-related protein synthesis, amino acid biosynthesis, nucleotide biosynthesis, sugar metabolism, transport systems, and vitamin biosynthesis. These findings provide a considerable background regarding the NaCl stress response of L. bulgaricus.
