Polymorphisms of IFS1 and IFS2 gene are associated with isoflavone concentrations in soybean seeds
Polymorphisms of IFS1 and IFS2 gene are associated with isoflavone concentrations in soybean seeds
复制标题
DOI：
10.1016/j.plantsci.2008.05.020
复制
发表时间：
2008-10-01
期刊：
影响因子：
5.2
通讯作者：
Yu, Deyue
中科院分区：
文献类型：
--
作者：
Cheng, Hao;Yu, Oliver;Yu, Deyue
大豆异黄酮与食用大豆的许多健康益处有关，异黄酮水平是大豆最重要的产量性状之一。异黄酮是由苯丙酸途径合成的。异黄酮合成酶（Isoflavone synthase， IFS）是各种异黄酮形成的关键代谢入口。我们对33份中国大豆材料的IFS1和IFS2基因组区进行了克隆、测序和分析，其中包括16份甘氨酸大豆和17份甘氨酸max大豆。异黄酮含量在536.6 μ g/g ~ 5509.1 μ g/g干粒重之间变化较大。这两个基因的高核苷酸多样性和低连锁不平衡程度（LD）为基因多态性与大豆种子异黄酮水平的关联分析提供了足够的遗传分辨率。结果表明，无论群体结构如何，IFS1基因的3个单核苷酸多态性（snp）和IFS2基因的2个单核苷酸多态性（snp）均与种子中所有个体和总异黄酮水平密切相关（P < 0.05）。这些结果表明，IFS1和IFS2基因都对种子中异黄酮的含量有影响。这些多态性可以作为育种的重要分子标记。2008爱思唯尔爱尔兰有限公司版权所有。
Soybean isoflavones are associated with many health benefits of soy consumption, and isoflavone levels are one of the most important output traits of soybean. Isoflavones are synthesized from the phenylpropanoid pathway. Isoflavone synthase (IFS) acts as the key metabolic entry point for the formation of all kinds of isoflavones. We have cloned, sequenced, and analyzed the IFS1 and IFS2 genomic regions from 33 Chinese soybean accessions including 16 Glycine soja and 17 Glycine max. The isoflavone levels in these accessions vary greatly, ranging from 536.6 mu g/g to 5509.1 mu g/g dry seed weight. High nucleotide diversity and low extent of linkage disequilibrium (LD) in these two genes provided sufficient genetic resolution for association analysis of polymorphisms in these genes and soybean seed isoflavone levels. As a result, three single nucleotide polymorphisms (SNPs) in IFS1 gene and two SNPs in IFS2 gene were found closely associated (P < 0.05) with all individual and total isoflavone levels in seeds, regardless of population structure. These results indicated that IFS1 and IFS2 gene both contributed to the levels of isoflavones in seeds. These polymorphisms may serve as important molecular markers for breeding. (c) 2008 Elsevier Ireland Ltd. All rights reserved.