Tracheal overexpression of IL-1β, IRAK-1 and TRAF-6 mRNA in obese-asthmatic male Wistar rats
Tracheal overexpression of IL-1β, IRAK-1 and TRAF-6 mRNA in obese-asthmatic male Wistar rats
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DOI：
10.22038/ijbms.2016.6805
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发表时间：
2016-04-01
影响因子：
2.2
通讯作者：
Alipour, Mohammad Reza
中科院分区：
文献类型：
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作者：
Aslani, Mohammad Reza;Keyhanmanesh, Rana;Alipour, Mohammad Reza
目的(S)：人类和动物研究表明，肥胖与哮喘严重程度密切相关。为探讨饮食诱导肥胖对雄性Wistar大鼠气管IL-1β、IRAK-1和TRAF-6mRNA表达的影响，将雄性Wistar大鼠20只随机分为4组，即正常饮食对照组(C+ND)、正常饮食致敏组(S+ND)、高脂饮食对照组(C+HFD)和高脂饮食S致敏组(+HFD)。所有动物用标准毛皮或高脂饲料喂养8周，然后按设计的方案致敏并用OVA或生理盐水激发4周。结果：饮食诱导肥胖组的体重、肥胖指数和血脂水平明显增加(P<；0.05~P<；0.001)。卵清蛋白致敏组(S+ND组和S+高脂汤组)IL-1β基因表达水平明显高于对照组。S+六味地黄汤组TRAF-6mRNA表达水平高于其他组(P<；0.001)。与对照组相比，S+肾衰汤组IRAK-1表达水平明显升高。IL-1β和TRAF-6mRNA与肥胖指数呈正相关。结论：DIO可导致实验性哮喘模型中IL-1β、IRAK-1和TRAF-6mRNA的过度表达。我们的结果表明，在肥胖-哮喘条件下，局部促炎因子的产生和激活可以增加。这些发现表明肥胖与哮喘的关系可能存在机制。
Objective(s): Human and animal studies have shown a close relationship between obesity and asthma severity. Here, we examined the effects of diet-induced obesity (DIO) on the expression levels of IL-1 beta, IRAK-1 and TRAF-6 mRNA in male Wistar rats tracheal after sensitization with ovalbumin (OVA).Materials and Methods: Twenty male Wistar rats divided to four groups, included, control group with normal diet (C+ND), OVA-sensitized group with normal diet (S+ND), control group with high-fat diet (C+HFD), and OVA-sensitized group with high-fat diet (S+HFD). All animals fed for 8 weeks with standard pelts or high-fat diet, and then were sensitized and challenged with OVA or saline for another 4 weeks with designed regimens. At the end of study, trachea isolated and examined for expression levels of IL-1 beta, IRAK-1 and TRAF-6 mRNA with RT-PCR method.Results: Diet-induced obesity groups developed increased weight, obesity indexes and lipid profiles (P< 0.05 to P< 0.001). The expression levels of IL-1 beta mRNA in OVA-sensitization groups (S+ND and S+HFD) showed a significantly increased when compared with control group. Also in S+HFD group, expression level of TRAF-6 mRNA was higher than other groups (P< 0.001). IRAK-1 expression level was high in S+HFD compared with control group. IL-1 beta and TRAF-6 mRNA correlated positively with obesity indexes.Conclusion: The results showed that DIO causes overexpression of IL-1 beta, IRAK-1 and TRAF-6 mRNA in an experimental model of asthma. Our results suggested that in obese-asthmatic conditions locally production and activation of pro-inflammatory agents can be increased. These findings showed that possible mechanism for obesity-asthma relationships.