Plasma hormone levels and central c-Fos expression in ferrets after systemic administration of cholecystokinin
Plasma hormone levels and central c-Fos expression in ferrets after systemic administration of cholecystokinin
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DOI：
10.1152/ajpregu.2001.281.4.r1243
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发表时间：
2001-10-01
影响因子：
2.8
通讯作者：
Rinaman, L
中科院分区：
文献类型：
--
作者：
Billig, I;Yates, BJ;Rinaman, L
研究了成年雪貂静脉注射CCK八肽后垂体后叶激素分泌和中枢神经即刻早期基因产物c-Fos的表达。CCK的药理学剂量（1、5、10或50马克杯/公斤）不会引起呕吐，但会引起恶心的行为症状和血浆抗利尿激素（AVP）水平的剂量相关升高，而血浆催产素（OT）水平没有显著升高。CCK激活了几个脑干脏器感觉区域的神经元c-Fos表达，包括背迷走神经复合体（DVC）神经元的剂量相关激活。激活的脑干神经元包括在DVC和腹外侧髓质的儿茶酚胺能和胰高血糖素样肽-1阳性细胞。在前脑，激活的神经元普遍存在于下丘脑室旁核和视上核，杏仁核中央核和终纹床核也有激活的神经元。激活的下丘脑神经元包括对AVP、OT和促肾上腺皮质激素释放因子有免疫反应的细胞。在雪貂腹腔注射经典催吐剂氯化锂（LiCl; 86 mg/kg）后，观察到脑干和前脑c-Fos的激活模式相似。然而，与CCK相比，LiCl激活了更多的后脑区神经元和更少的孤立束核神经元。结合先前在啮齿类动物中的研究结果，我们的发现支持了这样一种观点，即尽管治疗引起的呕吐和垂体激素分泌不同，但恶心治疗在呕吐和非呕吐物种中激活了相似的中枢神经回路。
Posterior pituitary hormone secretion and central neural expression of the immediate-early gene product c-Fos was examined in adult ferrets after intravenous administration of CCK octapeptide. Pharmacological doses of CCK (1, 5, 10, or 50 mug/kg) did not induce emesis, but elicited behavioral signs of nausea and dose-related increases in plasma vasopressin (AVP) levels without significant increases in plasma oxytocin (OT) levels. CCK activated neuronal c-Fos expression in several brain stem viscerosensory regions, including a dose-related activation of neurons in the dorsal vagal complex (DVC). Activated brain stem neurons included catecholaminergic and glucagon-like peptide-1-positive cells in the DVC and ventrolateral medulla. In the forebrain, activated neurons were prevalent in the paraventricular and supraoptic nuclei of the hypothalamus and also were observed in the central nucleus of the amygdala and bed nucleus of the stria terminalis. Activated hypothalamic neurons included cells that were immunoreactive for AVP, OT, and corticotropin-releasing factor. Comparable patterns of brain stem and forebrain c-Fos activation were observed in ferrets after intraperitoneal injection of lithium chloride (LiCl; 86 mg/kg), a classic emetic agent. However, LiCl activated more neurons in the area postrema and fewer neurons in the nucleus of the solitary tract compared with CCK. Together with results from previous studies in rodents, our findings support the view that nauseogenic treatments activate similar central neural circuits in emetic and nonemetic species, despite differences in treatment-induced emesis and pituitary hormone secretion.