Capillary-Mediated Single-Cell Dispenser.
Capillary-Mediated Single-Cell Dispenser.
复制标题
毛细管介导的单细胞分配器。
DOI：
10.1021/acs.analchem.1c01879
复制
发表时间：
2021
影响因子：
7.4
通讯作者：
Chiu,DanielT
中科院分区：
文献类型：
--
作者：
Wu,Li;Xu,Shihan;Wang,Jingang;Paguirigan,AmyL;Radich,JeraldP;Qin,Yuling;Chiu,DanielT
单细胞操作、分选和分配到多孔板中对于单细胞多组学研究是有用的。在这里，我们开发了一个单细胞分配器的灵感来自电流体动力学喷射打印，实现准确的液滴生成和单细胞分选和分配使用熔融石英毛细管作为光学检测窗口和喷嘴的液滴分配。影响微滴点胶性能的参数-毛细管内径和外径、流速、施加电压和溶液性质-通过COMSOL模拟和实验进行了系统优化。通过使用100-μm内径和160-μm外径的毛细管获得小（5-10 nL）液滴，并允许有效地包封和分配单细胞。我们展示了这种易于组装的单细胞分配器的应用，将细胞分选并分配到多孔板中进行单细胞PCR分析。
Single-cell manipulation, sorting, and dispensing into multiwell plates is useful for single-cell multiomics studies. Here, we develop a single-cell dispenser inspired by electrohydrodynamic jet printing that achieves accurate droplet generation and single-cell sorting and dispensing using fused silica capillary tubing as both the optical detection window and nozzle for droplet dispensing. Parameters that affect droplet dispensing performance—capillary inner and outer diameter, flow rate, applied voltage, and solution properties—were optimized systematically with COMSOL simulations and experimentation. Small (5–10 nL) droplets were obtained by using 100-μm inner diameter and 160-μm outer diameter capillary tubing and allowed efficient encapsulation and dispensing of single cells. We demonstrate an application of this easy-to-assemble single-cell dispenser by sorting and dispensing cells into multiwell plates for single-cell PCR analysis.