SCANNING LASER PHOTOSTIMULATION - A NEW APPROACH FOR ANALYZING BRAIN CIRCUITS
SCANNING LASER PHOTOSTIMULATION - A NEW APPROACH FOR ANALYZING BRAIN CIRCUITS
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DOI：
10.1016/0165-0270(94)90194-5
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发表时间：
1994-10-01
影响因子：
3
通讯作者：
DALVA, MB
中科院分区：
文献类型：
--
作者：
KATZ, LC;DALVA, MB
描述了一种用于理解脊椎动物大脑中复杂电路组织的新技术，扫描激光光刺激。这种方法是基于兴奋性神经递质谷氨酸的笼状形式的光解。计算机控制的光刺激和脑切片中的全细胞记录允许构建会聚在单个突触后神经元上的输入的位置、强度、符号和数量的详细地图。扫描激光光刺激提供了许多优于当前技术的优点：空间分辨率极佳，通道纤维不被激活，并且可以刺激数千个突触前位置。本文综述了光刺激技术，概述了必要的仪器来实现它，并讨论了光刺激衍生数据的解释。几个例子的应用，从映射电路在哺乳动物的视觉皮层，以确定受体分布在单个神经元被认为是。虽然仍处于早期阶段，但扫描激光光刺激为神经科学家提供了一个强大的工具，用于确定局部大脑回路的组织和功能。
A new technique for understanding the organization of complex circuits in the vertebrate brain, scanning laser photostimulation, is described. This approach is based on the photolysis of a caged form of the excitatory neurotransmitter glutamate. Computer-controlled photostimulation and whole cell recording in brain slices allow the construction of detailed maps of the position, strength, sign and number of inputs converging on a single postsynaptic neuron. Scanning laser photostimulation offers many advantages over current techniques: spatial resolution is superb, fibers of passage are not activated, and thousands of presynaptic locations can be stimulated. This review describes the technique of photostimulation, outlines the instrumentation necessary to implement it, and discusses the interpretation of photostimulation-derived data. Several examples of applications, ranging from mapping circuits in the mammalian visual cortex to determining receptor distributions on single neurons are considered. Although still in its early stages, scanning laser photostimulation offers neuroscientists a powerful tool for determining the organization and function of local brain circuits.