A novel 3D in vitro culture model to study stromal-epithelial interactions in the mammary gland
A novel 3D in vitro culture model to study stromal-epithelial interactions in the mammary gland
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DOI：
10.1089/ten.tec.2008.0030
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发表时间：
2008-09-01
影响因子：
3
通讯作者：
Sonnenschein, Carlos
中科院分区：
文献类型：
--
作者：
Krause, Silva;Maffini, Maricel V.;Sonnenschein, Carlos
基质-上皮相互作用介导乳腺发育和乳腺癌的形成和进展。为了在体外研究这些相互作用，定义的三维（3D）模型的发展是必不可少的。在本研究中，我们已经成功地开发了新的3D体外模型，使乳腺结构的形成非常类似于那些在体内发现的。将人乳腺上皮细胞系MCF 10A和人乳腺成纤维细胞共培养长达6周，所述人乳腺成纤维细胞得自包埋在I型胶原或混合的Matrigel-胶原基质中的还原乳房成形术。组织学和超微结构分析证实了导管和肺泡结构的形成。基质细胞的重要性在两种基质中是显而易见的;在胶原凝胶中，乳房成形术成纤维细胞减少的存在加速了上皮结构的初始形成，而在混合的基质胶-胶原凝胶中，这些成纤维细胞的存在对于导管结构的形成是必要的。这些模型提供了一个很好的系统来研究组织组织，上皮形态发生，和乳腺癌的发生。
Stromal-epithelial interactions mediate mammary gland development and the formation and progression of breast cancer. To study these interactions in vitro, the development of defined three-dimensional (3D) models is essential. In the present study, we have successfully developed novel 3D in vitro models that allow the formation of mammary gland structures closely resembling those found in vivo. Cocultures of a human mammary epithelial cell line MCF10A and human mammary fibroblasts obtained from reduction mammoplasties embedded in either a type I collagen or a mixed Matrigel-collagen matrix were carried out for up to 6 weeks. Histological and ultrastructural analysis confirmed the formation of ductal and alveolar structures. The importance of the stromal cells was apparent in both matrices; in the collagen gels the presence of reduction mammoplasty fibroblasts accelerated the initial formation of epithelial structures, and in the mixed Matrigel-collagen gels the presence of those fibroblasts was necessary for the formation of ductal structures. These models provide an excellent system to study tissue organization, epithelial morphogenesis, and breast carcinogenesis.