Establishment and characterization of human renal cancer and normal kidney cell lines.
Establishment and characterization of human renal cancer and normal kidney cell lines.
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发表时间：
1990-09
期刊：
影响因子：
11.2
通讯作者：
T. Ebert;N. Bander;C. L. Finstad;R. Ramsawak;Lloyd L. Old
中科院分区：
文献类型：
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作者：
T. Ebert;N. Bander;C. L. Finstad;R. Ramsawak;Lloyd L. Old
我们回顾了我们实验室为建立连续的人类肾癌细胞系所做的努力。在1972年至1987年的16年期间，共进行了498次连续尝试，最终建立了63种肾癌细胞系。在这些细胞系中，46个来自原发性肾肿瘤，17个来自转移部位（肺、脑、骨和淋巴结）。其中43个系在形态学、生长动力学、不依赖锚定生长、胸腺裸鼠的致瘤性和肾细胞表面抗原的表达方面具有特征。这些结果与正常肾上皮的初代短期培养数据进行了比较。建立连续肾癌细胞系的总成功率为12.7%。一般来说，根据标本是原发的还是转移的，成功率没有显著差异。然而，所有成功建立的细胞系都来源于表现出临床“侵袭性”行为的肿瘤。所有细胞系均表达近端小管细胞分化抗原。正常肾细胞株和肾癌细胞株在体外观察到明显的形态异质性。肾癌细胞系与正常肾上皮细胞传代培养在倍增时间上无显著差异。30个系中有21个（70%）在软琼脂上形成了克隆，33个系中有26个（79%）在胸腺小鼠中生长。在25个软琼脂生长和裸鼠致瘤性试验中，这对表型性状在17个（60%）系中一致。4株（16%）在琼脂上生长，但在小鼠体内不能生长，另外4株（16%）在琼脂上不能生长，但在小鼠体内具有致瘤性。
We have reviewed our laboratory's efforts to establish continuous human renal cancer cell lines. During the 16-year period of 1972 through 1987, 498 successive attempts resulted in establishment of 63 renal cancer cell lines. Of these lines, 46 were derived from primary kidney tumors and 17 from metastatic sites (lung, brain, bone, and lymph node). Forty-three of these lines have been characterized with regard to morphology, growth kinetics, anchorage-independent growth, tumorigenicity in athymic nude mice, and expression of kidney cell surface antigens. These results were compared with data from primary short term cultures of normal kidney epithelium. The overall success rate of establishing continuous renal cancer cell lines was 12.7%. In general, no significant difference in success was noted based on whether the specimen was derived from a primary or a metastatic lesion. However, all successfully established lines were derived from tumors exhibiting clinically "aggressive" behavior. All cell lines expressed proximal tubular cell differentiation antigens. Significant morphological heterogeneity was observed among normal kidney as well as kidney cancer cell lines in vitro. No significant difference in doubling time was found between cell lines of renal cancer and passage 1 cultures of normal kidney epithelium. Twenty-one of 30 (70%) lines assayed formed clones on soft agar and 26 of 33 (79%) lines grew in athymic mice. Among the 25 lines which were assayed for both soft agar growth and tumorigenicity in nude mice, this pair of phenotypic traits were concordant in 17 lines (60%). Four lines (16%) grew on agar but not in mice, while four other lines (16%) failed to grow in agar but were tumorigenic in mice.