The effects of continuous and discontinuous groove edges on cell shape and alignment
The effects of continuous and discontinuous groove edges on cell shape and alignment
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DOI：
10.1016/s0014-4827(03)00159-9
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发表时间：
2003-08-01
影响因子：
3.7
通讯作者：
Sutherland, D
中科院分区：
文献类型：
--
作者：
Andersson, AS;Olsson, P;Sutherland, D
纳米制造的模型表面和细胞形状的数字图像分析被用来解决细胞与浅表面凹槽对齐中连续锐边的重要性。开槽模型表面具有不同深度（40 - 400 nm）的连续或不连续边缘，但具有相同的表面化学性质和槽/脊尺寸（15 μ m宽）。将上皮细胞在模型表面上培养10和24 h。荧光显微镜结合图像分析用于量化细胞面积和排列，并对单个细胞进行细胞形状分类。在具有连续凹槽的样品上，细胞的排列程度和伸长细胞类的百分比随着凹槽深度而增加。两个主要的差异，关于细胞的反应，观察之间的连续和不连续的凹槽表面。首先，与具有连续边缘的凹槽相比，与具有不连续边缘的表面凹槽对齐的细胞显著更少。第二，有较低的百分比的不连续的凹槽比连续的细长细胞类。我们的结论是，具有连续边缘的凹槽表面在排列和诱导细长细胞方面更有效。本研究的结果表明，可能存在一种沿沿着连续边缘定向的对齐机制。(C)2003 Elsevier Science（美国）。All rights reserved.
Nanofabricated model surfaces and digital image analysis of cell shape were used to address the importance of a continuous sharp edge in the alignment of cells to shallow surface grooves. The grooved model surfaces had either continuous or discontinuous edges of various depths (40-400 nm) but identical surface chemistry and groove/ridge dimensions (15 mum wide). Epithelial cells were cultured on the model surfaces for 10 and 24 h. Fluorescence microscopy combined with image analysis were used to quantify cell area and alignment and to make cell shape classifications of individual cells. The degrees of alignment of cells and the percentages of elongated cell classes increased with groove depth on samples with continuous grooves. Two main differences, with regard to cell response, were observed between the continuous and discontinuous grooved surfaces. First, significantly fewer cells aligned to surface grooves with discontinuous edges than to grooves with continuous edges. Second, there were lower percentages of the elongated cell classes on discontinuous grooves than on continuous ones. We concluded that grooved surfaces with continuous edges are more potent in aligning and inducing elongated cells. The results from the present study suggest that a mechanism of alignment involving orientation along a continuous edge is likely. (C) 2003 Elsevier Science (USA). All rights reserved.