Compromising human skin in vivo and ex vivo to study skin barrier repair
Compromising human skin in vivo and ex vivo to study skin barrier repair
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DOI：
10.1016/j.bbalip.2019.04.005
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发表时间：
2019-08-01
影响因子：
4.8
通讯作者：
Bouwstra, J. A.
中科院分区：
文献类型：
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作者：
Berkers, T.;Boiten, W. A.;Bouwstra, J. A.
胶带剥离后的离体再生角质层（SC）可用作研究受损皮肤屏障功能的模型。然而，关于再生的SC模型如何接近模拟体内情况的脂质性质（例如脂质组成和脂质排序）的细节尚不清楚。在这里，我们研究了使用一个全面的神经酰胺分析是否人类离体再生SC显示出类似的脂质特性，作为人类在体内再生SC。在体内和离体再生SC有一个改变的神经酰胺亚类组成，增加的百分比的鞘氨醇为基础的亚类和减少的百分比的植物鞘氨醇为基础的亚类神经酰胺，减少的平均神经酰胺链长，和更高的百分比的不饱和神经酰胺。总体而言，再生SC离体显示出更明显的，但类似的变化相比，在体内的反应。这些模型的目的之一是使用它们来模拟炎症性皮肤疾病的受损皮肤。再生SC中改变的脂质性质与在几种炎性皮肤病中观察到的脂质性质相当，这使得它们成为炎性皮肤病中屏障性质的有价值的模型。
Ex vivo regenerated stratum corneum (SC) after tape-stripping can be used as a model to study the barrier function of compromised skin. Yet, details about how close the regenerated SC model mimics the lipid properties (e.g. lipid composition and lipid ordering) of the in vivo situation are not known. Here, we examined using a comprehensive ceramide analysis whether human ex vivo regenerated SC showed similar lipid properties as human in vivo regenerated SC. Both in vivo and ex vivo regenerated SC had an altered ceramide subclass composition, with increased percentages of sphingosine-based subclass and decreased percentages of phytosphingosine-based subclass ceramides, a reduced mean ceramide chain length, and a higher percentage of unsaturated ceramides. Overall, regenerated SC ex vivo showed more pronounced but similar changes compared to the in vivo response. One of the purposes of these models is to use them to mimic compromised skin of inflammatory skin diseases. The altered lipid properties in regenerated SC were comparable to those observed in several inflammatory skin diseases, which makes them a valuable model for the barrier properties in inflammatory skin diseases.